Department of Ecological Environment and Engineering, Yangling Vocational and Technical College, Shaanxi, Yangling, China.
Gansu Provincial Transportation Planning Survey and Design Institute Limited Liability Company, Lanzhou, Gansu Province, China.
PLoS One. 2024 Mar 21;19(3):e0299259. doi: 10.1371/journal.pone.0299259. eCollection 2024.
Temperature is one of the most important environmental factors for plant growth, as low-temperature freezing damage seriously affects the yield and distribution of plants. The Lanzhou lily (Lilium davidii, var. unicolor) is a famous ornamental plant with high ornamental value. Using an Illumina HiSeq transcriptome sequencing platform, sequencing was conducted on Lanzhou lilies exposed to two different temperature conditions: a normal temperature treatment at 20°C (A) and a cold treatment at -4°C (C). After being treated for 24 hours, a total of 5848 differentially expressed genes (DEGs) were identified, including 3478 significantly up regulated genes and 2370 significantly down regulated genes, accounting for 10.27% of the total number of DEGs. Quantitative real-time PCR (QRT-PCR) analysis showed that the expression trends of 10 randomly selected DEGs coincided with the results of high-throughput sequencing. In addition, genes responding to low-temperature stress were analyzed using the interaction regulatory network method. The anti-freeze pathway of Lanzhou lily was found to involve the photosynthetic and metabolic pathways, and the key freezing resistance genes were the OLEO3 gene, 9 CBF family genes, and C2H2 transcription factor c117817_g1 (ZFP). This lays the foundation for revealing the underlying mechanism of the molecular anti-freeze mechanism in Lanzhou lily.
温度是影响植物生长的最重要的环境因素之一，因为低温冷冻会严重影响植物的产量和分布。兰州百合（Lilium davidii，var. unicolor）是一种具有高观赏价值的著名观赏植物。本研究利用 Illumina HiSeq 转录组测序平台，对兰州百合在两种不同温度条件下进行测序：正常温度处理 20°C（A）和低温处理-4°C（C）。经过 24 小时处理后，共鉴定出 5848 个差异表达基因（DEGs），包括 3478 个显著上调基因和 2370 个显著下调基因，占 DEGs 总数的 10.27%。定量实时 PCR（QRT-PCR）分析显示，10 个随机选择的 DEGs 的表达趋势与高通量测序结果一致。此外，还通过互作调控网络方法分析了低温胁迫响应基因。发现兰州百合的抗冻途径涉及光合作用和代谢途径，关键抗冻基因是 OLEO3 基因、9 个 CBF 家族基因和 C2H2 转录因子 c117817_g1（ZFP）。这为揭示兰州百合分子抗冻机制的潜在机制奠定了基础。
