Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan 430062, China.
School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, China.
Food Chem. 2024 Jul 30;447:139056. doi: 10.1016/j.foodchem.2024.139056. Epub 2024 Mar 19.
Sinapic acid (SA), canolol (CAO) and canolol dimer (CAO dimer) are the main phenolic compounds in rapeseed oil. However, their possible efficacy against glycation remains unclear. This study aims to explore the impacts of these substances on the formation of advanced glycation end products (AGEs) based on chemical and cellular models in vitro. Based on fluorescence spectroscopy results, three chemical models of BSA-fructose, BSA-methylglyoxal (MGO), and arginine (Arg)-MGO showed that SA/CAO/CAO dimer could effectively reduce AGE formation but with different abilities. After SA/CAO/CAO dimer incubation, effective protection against BSA protein glycation was observed and three different MGO adducts were formed. In MGO-induced HUVEC cell models, only CAO and CAO dimer significantly inhibited oxidative stress and cell apoptosis, accompanied by the regulation of the Nrf2-HO-1 pathway. During the inhibition, 20 and 12 lipid mediators were reversed in the CAO and CAO dimer groups compared to the MGO group.
芥子酸（SA）、菜油醇（CAO）和菜油醇二聚体（CAO 二聚体）是菜籽油中的主要酚类化合物。然而，它们对糖化的可能疗效尚不清楚。本研究旨在基于体外化学和细胞模型探讨这些物质对晚期糖基化终产物（AGEs）形成的影响。根据荧光光谱结果，三种 BSA-果糖、BSA-甲基乙二醛（MGO）和精氨酸（Arg）-MGO 的化学模型表明，SA/CAO/CAO 二聚体可以有效减少 AGE 形成，但能力不同。SA/CAO/CAO 二聚体孵育后，观察到对 BSA 蛋白糖化的有效保护作用，并形成了三种不同的 MGO 加合物。在 MGO 诱导的 HUVEC 细胞模型中，只有 CAO 和 CAO 二聚体显著抑制氧化应激和细胞凋亡，并调节 Nrf2-HO-1 通路。在抑制过程中，与 MGO 组相比，CAO 和 CAO 二聚体组分别有 20 种和 12 种脂质介质被逆转。
