School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA.
Food Chem. 2016 Aug 15;205:204-11. doi: 10.1016/j.foodchem.2016.03.019. Epub 2016 Mar 8.
The objective of this study was to investigate the inhibitory effects of Camellia nitidissima Chi (CNC) on the advanced glycation end-product (AGE) formation. CNC was extracted with ethanol and further separated into dichloromethane, ethyl acetate, n-butanol, and water soluble fractions. Ethyl acetate fraction had the highest total phenolic and quercetin content compared with other fractions. Sixteen phenolic compounds were identified using HPLC Triple TOF MS/MS. Bovine serum albumin (BSA)-glucose assay showed that dichloromethane and ethyl acetate fraction inhibited AGE formation by 88.1% and 87.5% at 2.5mg/mL. BSA-methylglyoxal assay showed that ethyl acetate fraction inhibited 54.1% AGE formation while dichloromethane fraction inhibited 28.1%. Over 96.0% of methylglyoxal was scavenged by different fractions within 12h. Both mono- and di-methylglyoxal quercetin adducts were identified after incubating quercetin with methylglyoxal using HPLC-ESI-MS(n). The results in this study suggest that CNC extracts inhibited AGEs formation in part through scavenging methylglyoxal by phenolic compounds.
本研究旨在探讨金花茶（CNC）对糖基化终产物（AGE）形成的抑制作用。采用乙醇提取金花茶，并进一步分离为二氯甲烷、乙酸乙酯、正丁醇和水溶性馏分。与其他馏分相比，乙酸乙酯馏分的总酚和槲皮素含量最高。采用 HPLC Triple TOF MS/MS 鉴定了 16 种酚类化合物。牛血清白蛋白（BSA）-葡萄糖测定表明，二氯甲烷和乙酸乙酯馏分在 2.5mg/mL 时抑制 AGE 形成率分别为 88.1%和 87.5%。BSA-甲基乙二醛测定表明，乙酸乙酯馏分抑制 54.1%的 AGE 形成，而二氯甲烷馏分抑制 28.1%。不同馏分在 12 小时内可清除超过 96.0%的甲基乙二醛。用 HPLC-ESI-MS(n) 检测到在用甲基乙二醛孵育槲皮素后形成的单-和二-甲基乙二醛槲皮素加合物。本研究结果表明，金花茶提取物通过酚类化合物清除甲基乙二醛部分抑制 AGEs 的形成。
