一种用于创建新型遗传网络拓扑结构的模块化转录调控因子的结构域交换策略。
A domain swapping strategy to create modular transcriptional regulators for novel topology in genetic network.
机构信息
Department of Biomedical Engineering, University of North Texas, TX 76207, USA; BioDiscovery Institute, University of North Texas, TX 76207, USA.
Department of Biomedical Engineering, University of North Texas, TX 76207, USA.
出版信息
Biotechnol Adv. 2024 May-Jun;72:108345. doi: 10.1016/j.biotechadv.2024.108345. Epub 2024 Mar 19.
Transcriptional regulators generate connections between biological signals and genetic outputs. They are used robustly for sensing input signals in building genetic circuits. However, each regulator can only generate a fixed connection, which generates constraints in linking multiple signals for more complex processes. Recent studies discovered that a domain swapping strategy can be applied to various regulator families to create modular regulators for new signal-output connections, significantly broadening possibilities in circuit design. Here we review the development of this emerging strategy, the use of resulting modular regulators for creating novel genetic response behaviors, and current limitations and solutions for further advancing the design of modular regulators.
转录调控因子在生物信号和基因表达之间建立联系。它们在构建基因回路时被广泛用于感知输入信号。然而，每个调控因子只能产生固定的连接，这在将多个信号连接起来以实现更复杂的过程时会产生限制。最近的研究发现，一种结构域交换策略可以应用于各种调控因子家族，从而为新的信号-输出连接创建模块化的调控因子，极大地拓宽了电路设计的可能性。本文综述了这一新兴策略的发展，以及使用由此产生的模块化调控因子来创建新的遗传反应行为的情况，并讨论了进一步推进模块化调控因子设计的当前限制和解决方案。
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