Biomedical Engineering Centre, Institute of Optoelectronics, Military University of Technology, Kaliskiego, Warsaw, Poland.
Laser Technology Division, Institute of Optoelectronics, Military University of Technology, Kaliskiego, Warsaw, Poland.
Bioessays. 2024 May;46(5):e2300122. doi: 10.1002/bies.202300122. Epub 2024 Mar 21.
Fluorescence microscopy is a powerful tool used in scientific and medical research, but it is inextricably linked to phototoxicity. Neglecting phototoxicity can lead to erroneous or inconclusive results. Recently, several reports have addressed this issue, but it is still underestimated by many researchers, even though it can lead to cell death. Phototoxicity can be reduced by appropriate microscopic techniques and carefully designed experiments. This review focuses on recent strategies to reduce phototoxicity in microscopic imaging of living cells and tissues. We describe digital image processing and new hardware solutions. We point out new modifications of microscopy methods and hope that this review will interest microscopy hardware engineers. Our aim is to underscore the challenges and potential solutions integral to the design of microscopy systems. Simultaneously, we intend to engage biologists, offering insight into the latest technological advancements in imaging that can enhance their understanding and practice.
荧光显微镜是科学和医学研究中使用的一种强大工具，但它与光毒性密不可分。忽视光毒性可能会导致错误或不确定的结果。最近，有几项报告已经解决了这个问题，但许多研究人员仍然低估了这个问题，尽管它可能导致细胞死亡。通过适当的显微镜技术和精心设计的实验，可以减少光毒性。本综述重点介绍了最近在活细胞和组织的显微镜成像中减少光毒性的策略。我们描述了数字图像处理和新的硬件解决方案。我们指出了显微镜方法的新修改，并希望本综述能引起显微镜硬件工程师的兴趣。我们的目的是强调显微镜系统设计中不可或缺的挑战和潜在解决方案。同时，我们希望让生物学家参与进来，了解可以增强他们理解和实践的成像领域的最新技术进展。
