Poupin F, Cailliez M, Carrie C, Petithory J C, Savel J
Bull Soc Pathol Exot Filiales. 1978 Nov-Dec;71(6):430-40.
The enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of human african trypanosomiasis is described, comparatively with the immunoperoxidase technique using blood streamforms of Trypanosoma brucei as antigen. Practical aspects for measuring the amount of antibody in serum samples from infected rabbits and from patients with african sleeping sickness are discussed. ELISA does offer important advantages over the other method used namely: -- quantitative estimates are possible, -- it bends itself readily to large scale series, owing the fact that it can be fully automatic. These qualities will be turned to full account when the different classes of antibodies present in trypanosomiasis suffers can be examined by means of specific antigenic fractions.
本文描述了用于人类非洲锥虫病血清诊断的酶联免疫吸附测定（ELISA），并与以布氏锥虫血流型为抗原的免疫过氧化物酶技术进行了比较。讨论了测定感染兔子和非洲昏睡病患者血清样本中抗体量的实际问题。ELISA相对于所使用的其他方法确实具有重要优势，即：——可以进行定量估计；——由于它可以完全自动化，因此很容易适用于大规模检测。当通过特定抗原组分检测锥虫病患者体内存在的不同类别抗体时，这些特性将得到充分利用。
