Liu M K, Pearson T W, Sayer P D, Gould S S, Waitumbi J N, Njogu A R
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Acta Trop. 1988 Dec;45(4):321-30.
Sera of vervet monkeys experimentally infected with T. b. rhodesiense were examined using a double antibody sandwich ELISA and Procyclic Agglutination Trypanosomiasis Test (PATT) for the presence of circulating trypanosomal antigens and anti-procyclic surface antibodies, respectively. Trypanosomal antigens were detected at 7 days post infection and remained at a detectable level thereafter during the infection. Antigens were not detected in sera prior to experimental infection or at 26 days after trypanocidal drug treatment. Although both the PATT and the sandwich ELISA results correlated with the infection status of the animals, the sandwich ELISA gave a better indication of the disease progression than the PATT, especially during trypanocidal drug therapy. The results illustrate the potential utility of the double antibody sandwich ELISA for diagnosis of African sleeping sickness.
使用双抗体夹心酶联免疫吸附测定（ELISA）和前环状体凝集锥虫病试验（PATT），分别检测实验感染罗德西亚锥虫的绿猴血清中循环锥虫抗原和抗前环状体表面抗体的存在情况。感染后7天检测到锥虫抗原，此后在感染期间一直保持在可检测水平。在实验感染前或锥虫杀灭药物治疗后26天的血清中未检测到抗原。虽然PATT和夹心ELISA的结果都与动物的感染状况相关，但夹心ELISA比PATT更能较好地指示疾病进展，尤其是在锥虫杀灭药物治疗期间。结果表明双抗体夹心ELISA在非洲昏睡病诊断中的潜在应用价值。
