皮质醇荧光偏振免疫测定法

Fluorescence polarization immunoassay for cortisol.

作者信息

Kobayashi Y, Amitani K, Watanabe F, Miyai K

出版信息

Clin Chim Acta. 1979 Mar 1;92(2):241-7. doi: 10.1016/0009-8981(79)90119-0.

Abstract

A fluorescence polarization immunoassay for serum cortisol was established using cortisol 21-amine which was readily coupled with fluorescein isothiocyanate. The proposed method is sufficiently sensitive, reliable, specific and simple for routine determination of serum cortisol. The assay is rapid, without separation of antibody-bound and free ligands. The minimal amount of cortisol detected was 1.5 ng/tube and the measurable range was from 1.5 to 100 micrograms/dl. There was a good correlation between values obtained from radioimmunoassay and the proposed method.

摘要

利用易于与异硫氰酸荧光素偶联的皮质醇 21 - 胺建立了一种用于血清皮质醇的荧光偏振免疫测定法。所提出的方法对于血清皮质醇的常规测定具有足够的灵敏度、可靠性、特异性且操作简单。该测定法快速，无需分离抗体结合的和游离的配体。检测到的皮质醇最小量为 1.5 ng/管，可测量范围为 1.5 至 100 μg/dl。放射免疫测定法获得的值与所提出的方法之间具有良好的相关性。