Halat Monika, Zając Grzegorz, Andrushchenko Valery, Bouř Petr, Baranski Rafal, Pajor Katarzyna, Baranska Malgorzata
Department of Plant Biology and Biotechnology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120, Krakow, Poland.
Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
Angew Chem Int Ed Engl. 2024 May 21;63(21):e202402449. doi: 10.1002/anie.202402449. Epub 2024 Apr 12.
Carotenoids tend to form supramolecular aggregates via non-covalent interactions where the chirality of individual molecules is amplified to the macroscopic level. We show that this can also be achieved for non-chiral carotenoid monomers interacting with polysaccharides. The chirality induction in canthaxanthin (CAX), caused by heparin (HP) and hyaluronic acid (HA), was monitored by chiroptical spectroscopy. Electronic circular dichroism (ECD) and Raman optical activity (ROA) spectra indicated the presence of multiple carotenoid formations, such as H- and J-type aggregates. This is consistent with molecular dynamics (MD) and density functional theory (DFT) simulations of the supramolecular structures and their spectroscopic response.
类胡萝卜素倾向于通过非共价相互作用形成超分子聚集体，其中单个分子的手性被放大到宏观水平。我们表明，对于与多糖相互作用的非手性类胡萝卜素单体，也可以实现这一点。通过手性光谱监测了由肝素（HP）和透明质酸（HA）引起的角黄素（CAX）中的手性诱导。电子圆二色性（ECD）和拉曼光学活性（ROA）光谱表明存在多种类胡萝卜素结构，如H型和J型聚集体。这与超分子结构及其光谱响应的分子动力学（MD）和密度泛函理论（DFT）模拟结果一致。
