State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; State Environmental Protection Key Laboratory of Hazardous Waste Identification and Risk Control, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; College of Life Science, Northeast Agricultural University, Harbin, 150030, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; State Environmental Protection Key Laboratory of Hazardous Waste Identification and Risk Control, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China; School of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541000, China.
Environ Res. 2024 Jul 1;252(Pt 1):118724. doi: 10.1016/j.envres.2024.118724. Epub 2024 Mar 20.
The interactive effects between the emerging contaminant antibiotic resistance genes (ARGs) and the traditional pollutant total petroleum hydrocarbons (TPHs) in contaminated soils remain unclear. The synergistic removal of TPHs and ARGs from composted contaminated soil, along with the microbial mechanisms driven by the addition of biogas slurry, have not yet been investigated. This study explored the impact of biogas slurry on the synergistic degradation mechanisms and bacterial community dynamics of ARGs and TPHs in compost derived from contaminated soil. The addition of biogas slurry resulted in a reduction of targeted ARGs and mobile genetic elements (MGEs) by 9.96%-95.70% and 13.32%-97.66%, respectively. Biogas slurry changed the succession of bacterial communities during composting, thereby reducing the transmission risk of ARGs. Pseudomonas, Cellvibrio, and Devosia were identified as core microorganisms in the synergistic degradation of ARGs and TPHs. According to the partial least squares path model, temperature and NO indirectly influenced the removal of ARGs and TPHs by directly regulating the abundance and composition of host microbes and MGEs. In summary, the results of this study contribute to the high-value utilization of biogas slurry and provide methodological support for the low-cost remediation of contaminated soils.
新兴污染物抗生素抗性基因（ARGs）与传统污染物总石油烃（TPHs）在污染土壤中的相互作用尚不清楚。尚未研究过沼气液对堆肥污染土壤中 ARGs 和 TPHs 的协同去除及其微生物机制。本研究探讨了沼气液对污染土壤源堆肥中 ARGs 和 TPHs 协同降解机制及细菌群落动态的影响。添加沼气液可使目标 ARGs 和移动遗传元件（MGEs）分别减少 9.96%-95.70%和 13.32%-97.66%。沼气液改变了堆肥过程中细菌群落的演替，从而降低了 ARGs 的传播风险。假单胞菌、纤维弧菌和德沃斯氏菌被鉴定为 ARGs 和 TPHs 协同降解的核心微生物。根据偏最小二乘路径模型，温度和 NO 通过直接调节宿主微生物和 MGEs 的丰度和组成间接影响 ARGs 和 TPHs 的去除。总之，本研究结果为沼气液的高值化利用提供了依据，为污染土壤的低成本修复提供了方法学支持。
