College of Resources and Environment, Hunan Agricultural University, Changsha 410128, Hunan, China.
POWERCHINA Zhongnan Engineering Corporation Limited, Changsha 410014, Hunan, China.
Bioresour Technol. 2023 Mar;372:128636. doi: 10.1016/j.biortech.2023.128636. Epub 2023 Jan 16.
This research investigated biogas residue and biochar addition on antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and changes in bacterial community during agricultural waste composting. Sequencing technique investigated bacterial community structure and ARGs, MGEs changes. Correlations among physicochemical factors, ARGs, MGEs, and bacterial community structure were determined using redundancy analysis. Results confirmed that biochar and biogas residue amendments effectively lowered the contents of ARGs and MGEs. The main ARGs detected was sul1. Proteobacteria and Firmicutes were the main host bacteria strongly associated with the dissemination of ARGs. The dynamic characteristics of the bacterial community were strongly correlated with pile temperature and pH (P < 0.05). Redundancy and network analysis revealed that nitrate, intI1, and Firmicutes mainly affected the in ARGs changes. Therefore, regulating these key variables would effectively suppress the ARGs spread and risk of compost use.
本研究调查了沼气残渣和生物炭添加对农业废弃物堆肥过程中抗生素抗性基因（ARGs）、移动遗传元件（MGEs）和细菌群落变化的影响。测序技术研究了细菌群落结构和 ARGs、MGEs 的变化。冗余分析确定了理化因子、ARGs、MGEs 和细菌群落结构之间的相关性。结果证实，生物炭和沼气残渣的添加有效地降低了 ARGs 和 MGEs 的含量。检测到的主要 ARGs 是 sul1。变形菌门和厚壁菌门是与 ARGs 传播密切相关的主要宿主菌。细菌群落的动态特征与堆温（P<0.05）和 pH 值密切相关。冗余和网络分析表明，硝酸盐、intI1 和厚壁菌门主要影响 ARGs 的变化。因此，调节这些关键变量将有效地抑制 ARGs 的传播和堆肥使用的风险。
