College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China.
College of Fisheries, Henan Normal University, Xinxiang 453007, Henan, China.
Food Res Int. 2024 Apr;182:114140. doi: 10.1016/j.foodres.2024.114140. Epub 2024 Feb 18.
DNA-based methods are reliable for a precise identification of species in processed products. In this study, we assessed five typical DNA extraction methods from multiple aspects. Full-length and mini-length DNA barcoding were performed to detect the species substitution and mislabeling of 305 processed fish products from the Chinese market covering six processed fish products. The salt extraction method that exhibited the best overall performance was applied. All samples were successfully extracted; however, only 19.3 % of samples could be amplified using the full-DNA barcode primer set, and 90.2 % of samples could be amplified using the newly designed mini-DNA barcode primer sets (401 and 320 bp). Overall, the molecular identification results revealed that 36.4 % (111/305) of the samples were inconsistent with the labels, with commercial fraud observed in all six types of processed fish products. The survey findings provide technical references for effective fish authentication monitoring, offering insights into the seafood safety in markets.
基于 DNA 的方法可用于可靠地鉴定加工产品中的物种。在这项研究中，我们从多个方面评估了五种典型的 DNA 提取方法。对来自中国市场的 305 种加工鱼类产品（涵盖 6 种加工鱼类产品）进行全长和 mini-length DNA 条形码检测，以检测物种替代和标签错误。采用表现出最佳整体性能的盐提取方法。所有样品均成功提取，但仅使用全长 DNA 条码引物组可扩增 19.3%的样品，而使用新设计的 mini-DNA 条码引物组（401 和 320 bp）可扩增 90.2%的样品。总体而言，分子鉴定结果显示，36.4%（111/305）的样品与标签不符，在所有 6 种加工鱼类产品中都存在商业欺诈行为。调查结果为有效鱼类鉴定监测提供了技术参考，为市场海鲜安全提供了新的见解。
