DNA 条形码和微条形码在鉴定加工动物源性食品中的应用：以中国市场为例的研究。
DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market.
机构信息
Agro-product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Agro-product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100176, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
出版信息
Food Chem. 2020 Mar 30;309:125653. doi: 10.1016/j.foodchem.2019.125653. Epub 2019 Oct 16.
This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (650 bp) and 16S rRNA (220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI-failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted.
本研究采用 DNA 条码和 DNA 微条码技术，对中国市场上销售的各种动物源性食品进行潜在的标签错误检测。检测了从零售和网络渠道购买的 52 个肉类、禽类和鱼类样本。细胞色素 C 氧化酶 I（COI）基因（约 650bp）和 16S rRNA（约 220bp）区域分别作为全条码和微条码标记。大约 94%（52 个样本中的 49 个）的样本生成了条码序列。COI 全条码的失败率为 44%，但我们从 87%的 COI 失败案例中获得了 16S rRNA 微条码。总体而言，调查显示 23%（52 个样本中的 12 个）的动物源性产品存在标签错误，且在大多数情况下，包含未申报的物种。因此，应该对动物源性产品的标签错误进行监管措施和持续监测。
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