巴西有和没有 COVID-19 的患者携带 blaKPC 和 blaNDM 的毒力肺炎克雷伯菌 ST11 克隆。
Virulent Klebsiella pneumoniae ST11 clone carrying blaKPC and blaNDM from patients with and without COVID-19 in Brazil.
机构信息
Universidade Federal de Pernambuco-UFPE, Laboratório de Microbiologia, Área de Medicina Tropical, Centro de Ciências Médicas-CCM, 50670-901, Recife-PE, Brasil.
Secretaria de Saúde do Estado de Pernambuco, 50751-230, Recife-PE, Brazil.
出版信息
J Appl Microbiol. 2024 Apr 1;135(4). doi: 10.1093/jambio/lxae079.
AIMS
Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA, and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil.
METHODS AND RESULTS
All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and MLST were used. The detection of blaNDM was greater (n = 23) when compared to that of blaKPC (n = 14). The virulence genes that most occurred were fimH, entB, cps, and wabG, which are responsible for adhesins, siderophore enterobactin, capsule, and lipopolysaccharides, respectively. Among the isolates, 21 distinct genetic profiles were found by ERIC-PCR, with multiclonal dissemination. Four isolates belonged to the ST11 clone.
CONCLUSIONS
The occurrence of the ST11 is worrying as it is a high-risk clone involved in the dissemination of virulent strains throughout the world.
目的
调查和比较巴西一家医院的 29 例新冠肺炎患者和非新冠肺炎患者的肺炎克雷伯菌临床分离株中毒力基因 fimH、mrkD、irp2、entB、cps、rmpA 和 wabG、耐药基因 blaKPC 和 blaNDM 的发生率，以及它们的遗传变异和克隆关系。
方法和结果
所有分离株均对β-内酰胺类药物耐药。通过 PCR 检测基因，采用肠杆菌基因间重复一致性聚合酶链反应（ERIC-PCR）和 MLST 进行分子分型。blaNDM 的检出率（n=23）高于 blaKPC（n=14）。最常发生的毒力基因是 fimH、entB、cps 和 wabG，分别负责黏附素、铁载体 enterobactin、荚膜和脂多糖。通过 ERIC-PCR 发现 21 种不同的遗传谱，存在多克隆传播。有 4 株分离株属于 ST11 克隆。
结论
ST11 的发生令人担忧，因为它是一种高风险克隆，与世界各地毒力株的传播有关。
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