Universidade Federal de Pernambuco, Programa de Pós-Graduação em Medicina Tropical, Recife, PE, 50670-901, Brasil.
Secretaria Estadual de Saúde de Pernambuco, Recife, PE, 50751-530, Brasil.
J Appl Microbiol. 2024 Aug 5;135(8). doi: 10.1093/jambio/lxae212.
This study aimed to investigate the presence of beta-lactams resistance genes and the clonal relationship of clinical isolates of Enterobacterales obtained from patients with and without COVID-19, in a hospital in northeastern Brazil.
The study analyzed 45 carbapenem-resistant clinical isolates using enterobacterial repetitive intergenic consensus (ERIC-PCR), PCR, and amplicon sequencing to detect resistance genes (blaKPC, blaGES, blaNDM, blaVIM, and blaIMP). The main species were Klebsiella pneumoniae, Serratia marcescens, and Proteus mirabilis. Detected genes included blaNDM (46.66%), blaKPC (35.55%), and both (17.79%). ERIC-PCR showed multiclonal dissemination and high genetic variability. The main resistance gene was blaNDM, including blaNDM-5 and blaNDM-7.
The presence of Enterobacterales carrying blaKPC and blaNDM in this study, particularly K. pneumoniae, in infections and colonizations of patients with COVID-19 and non-COVID-19, highlights genetic variability and resistance to carbapenems observed in multiple species of this order.
本研究旨在调查来自巴西东北部一家医院的 COVID-19 患者和非 COVID-19 患者的肠杆菌目临床分离株中β-内酰胺类耐药基因的存在情况及其克隆关系。
该研究使用肠杆菌基因间重复一致序列（ERIC-PCR）、PCR 和扩增子测序分析了 45 株碳青霉烯类耐药的临床分离株，以检测耐药基因（blaKPC、blaGES、blaNDM、blaVIM 和 blaIMP）。主要分离株为肺炎克雷伯菌、粘质沙雷氏菌和奇异变形杆菌。检测到的基因包括 blaNDM（46.66%）、blaKPC（35.55%）和两者均携带（17.79%）。ERIC-PCR 显示了多克隆传播和高遗传变异性。主要耐药基因是 blaNDM，包括 blaNDM-5 和 blaNDM-7。
本研究中，携带 blaKPC 和 blaNDM 的肠杆菌目，尤其是肺炎克雷伯菌，在 COVID-19 和非 COVID-19 患者的感染和定植中存在，这突显了该菌目中多种物种存在的遗传变异性和对碳青霉烯类的耐药性。
