Molecular Diagnostics Team, Genes Laboratories, 388, Dunchon-daero, Jungwon-gu, Seongnam-si, Gyeonggi-do, 13403, Republic of Korea.
College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Arch Virol. 2024 Mar 23;169(4):82. doi: 10.1007/s00705-024-06003-9.
Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.
猪流行性腹泻病毒（PEDV）、猪德尔塔冠状病毒（PDCoV）和猪急性腹泻综合征冠状病毒（SADS-CoV）均可引起哺乳仔猪具有相似临床表现的肠道疾病。本研究中，我们建立了一种可用于 PEDV、PDCoV 和 SADS-CoV 鉴别诊断的多重实时 PCR 方法。该方法具有高特异性，其对每种病毒的检测限为 5 拷贝/μl。该方法可特异性检测 PEDV、PDCoV 和 SADS-CoV，且可排除猪群中流行的其他所有猪源病原体。此外，该方法在分析临床样本时表现出良好的性能。数据表明，新建立的多重实时 PCR 方法可用于猪肠道冠状病毒的鉴别诊断。
