Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Shenzhen Research Institute, Southeast University, Shenzhen, 518038, China.
Adv Sci (Weinh). 2024 Jun;11(22):e2400189. doi: 10.1002/advs.202400189. Epub 2024 Mar 23.
Anticoagulation is vital to maintain blood fluidic status and physiological functions in the field of clinical blood-related procedures. Here, novel biomimetic anticoagulated porous inverse opal hydrogel particles is presented as anticoagulant bearing dynamic screening capability. The inverse opal hydrogel particles possess abundant sulfonic and carboxyl groups, which serve as binding sites with multiple coagulation factors and inhibit the blood coagulation process. Owing to the variations of refractive index and pore sizes during the binding process, the particles appeared corresponding structure color variations, which can be adopted as sensory index of anticoagulation. Based on these features, a sensor containing these diverse structure color particle units is constructed for pattern recognition of coagulation factors level in clinical plasma samples. By analyzing the sensory information of the unit, the colorimetric "fingerprint" for each target can be obtained and summarized as a database. Besides, a portable test-strip integrating sensory units is developed to distinguish the sample regarding abnormal coagulation factors-derived diseases via multivariate data analysis. It is believed that such biomimetic anticoagulated structural color particles and their derived sensor will open new avenue for clinical detection and disease diagnosis.
抗凝在临床血液相关操作领域对于维持血液流态和生理功能至关重要。在此，我们提出了一种新型仿生抗凝多孔反蛋白石水凝胶颗粒，作为具有动态筛选能力的抗凝剂。反蛋白石水凝胶颗粒具有丰富的磺酸基和羧基，作为与多种凝血因子结合的位点，可抑制血液凝固过程。由于在结合过程中折射率和孔径的变化，颗粒会出现相应的结构颜色变化，可作为抗凝的感应指标。基于这些特性，构建了一种包含这些不同结构色颗粒单元的传感器，用于对临床血浆样本中凝血因子水平进行模式识别。通过分析单元的感应信息，可以获得每个目标的比色“指纹”，并将其总结为数据库。此外，还开发了一种集成感应单元的便携式测试条，通过多元数据分析来区分异常凝血因子相关疾病的样本。我们相信，这种仿生抗凝结构色颗粒及其衍生的传感器将为临床检测和疾病诊断开辟新的途径。
