Departments of Infectious Disease, Military Hospital 103, 261 Phung Hung, Ha Dong, Hanoi, Viet Nam.
Luong the Vinh High School, 35 Dinh Nup, Trung Hoa, Cau Giay, Hanoi, Viet Nam.
Virology. 2024 Jun;594:110058. doi: 10.1016/j.virol.2024.110058. Epub 2024 Mar 16.
HPV16, with typical mutations that differ in geographical distribution and carcinogenic potency, has implications for cervical cancer screening, clinical diagnosis, and treatment. DNASTAR and MEGA were used to identify HPV16 variants and construct a phylogenetic tree. The most prevalent HPV genotypes were HPV16 (63.9%), HPV18 (26.7%), and other HPV (6.9%). HPV16 alterations were found in all E6, E7, and L1 genes, including 15 missense and 18 synonymous mutations. Missense mutations include R10G, Q14H, D25E, H78Y, L83V (E6); M29V, R35K, L78R, L95P (E7); H73Y, T176 N, N178T, T317P, T386S, L472F/I (L1). HPV16 sublineages include A1 (17.2%), A2 (0.9%), A3 (56.0%), A4 (19.0%), D1 (4.3%), and D3 (2.6%). Although several mutations in the oncoproteins E6, E7, and L1 have been detected, mutations known to be associated with cervical cancer risk, such as D25E and L83V, occur at a relatively low frequency. This suggests that HPV16 mutations are associated with cervical cancer through a complicated mechanism.
HPV16 具有典型的突变，这些突变在地理分布和致癌能力上有所不同，这对宫颈癌筛查、临床诊断和治疗具有重要意义。我们使用 DNASTAR 和 MEGA 来鉴定 HPV16 变异体并构建系统发生树。最常见的 HPV 基因型是 HPV16（63.9%）、HPV18（26.7%）和其他 HPV（6.9%）。在所有的 E6、E7 和 L1 基因中均发现 HPV16 改变，包括 15 个错义突变和 18 个同义突变。错义突变包括 R10G、Q14H、D25E、H78Y、L83V（E6）；M29V、R35K、L78R、L95P（E7）；H73Y、T176N、N178T、T317P、T386S、L472F/I（L1）。HPV16 亚谱系包括 A1（17.2%）、A2（0.9%）、A3（56.0%）、A4（19.0%）、D1（4.3%）和 D3（2.6%）。尽管已经检测到了几个致癌蛋白 E6、E7 和 L1 中的突变，但与宫颈癌风险相关的突变，如 D25E 和 L83V，发生的频率相对较低。这表明 HPV16 突变与宫颈癌的发生是通过复杂的机制相关联的。
