雌激素和孕激素对牛输卵管上皮细胞内游离锌和锌转运体表达的影响。
Effect of estrogen and progesterone on intracellular free zinc and zinc transporter expression in bovine oviduct epithelial cells.
机构信息
IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.
IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina; YPF Tecnología (Y-TEC), Av. Del Petróleo S/N entre 129 y 143, CP 1923, Berisso, Buenos Aires, Argentina.
出版信息
Theriogenology. 2024 Jun;221:18-24. doi: 10.1016/j.theriogenology.2024.03.011. Epub 2024 Mar 19.
Zinc (Zn) plays essential roles in numerous cellular processes. However, there is limited understanding of Zn homeostasis within the bovine reproductive system. This study investigated the influence of estradiol (E) and progesterone (P) on Zn transporter expression and intracellular free Zn levels in bovine oviduct epithelial cells (BOEC). For this purpose, cells were harvested from slaughtered cows and cultured in vitro. Intracellular Zn concentrations were measured using FluoZin-3AM staining, while real-time polymerase chain reaction assessed Zn transporter gene expression and quantification. Overall, our results confirmed the gene expression of all the evaluated Zn transporters (ZIP6, ZIP8, ZIP14, ZnT3, ZnT7 and ZnT9), denoted and the active role of E and P in intracellular Zn regulation. Our findings suggest an interaction between Zn, E and P
锌 (Zn) 在许多细胞过程中发挥着重要作用。然而，人们对牛生殖系统中的锌稳态知之甚少。本研究探讨了雌二醇 (E) 和孕酮 (P) 对牛输卵管上皮细胞 (BOEC) 中锌转运体表达和细胞内游离锌水平的影响。为此，从屠宰牛中采集细胞并进行体外培养。使用 FluoZin-3AM 染色测量细胞内锌浓度，实时聚合酶链反应评估锌转运体基因表达和定量。总的来说，我们的结果证实了所有评估的锌转运体 (ZIP6、ZIP8、ZIP14、ZnT3、ZnT7 和 ZnT9) 的基因表达，并表明 E 和 P 在细胞内锌调节中的积极作用。我们的发现表明 Zn、E 和 P 之间存在相互作用。
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