College of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou 450001, China.
College of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou 450001, China.
Int J Pharm. 2024 Apr 25;655:124030. doi: 10.1016/j.ijpharm.2024.124030. Epub 2024 Mar 21.
Disease-causing microorganisms such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are among the primary contributors to morbidity and mortality of diarrhea in humans. Considering the challenges associated with antibiotic use, including antimicrobial resistance, this study aimed to develop a novel zinc-based agent for bacterial inactivation. To this end, zinc caproate (ZnCA) was synthesized using caproic acid (CA) and zinc oxide (ZnO) in anhydrous ethanol via the solvothermal method. Structural characterization techniques, including Fourier-transform infrared spectroscopy, single crystal X-ray diffraction analysis, and nuclear magnetic resonance spectroscopy, revealed the bidentate bridging coordination of zinc atoms with CA. The resulting two-dimensional ZnCA network was found to be composed of a distinct lamellar pattern, without any evident inter-layer interactions. Powder X-ray diffraction analysis, elemental analysis, and melting point analysis confirmed that ZnCA had an average particle size of 1.320 µm, a melting point of 147.2 °C, and a purity exceeding 98 %. Remarkably, ZnCA demonstrated potent antibacterial activity against E. coli and S. aureus, which exceeded the antibacterial efficacy of ZnO. ZnCA exerted its antibacterial effects by inhibiting biofilm formation, disrupting cell membrane integrity, increasing cell membrane permeability, and altering intracellular Ca-Mg-ATPase activity. These findings highlight the potential of ZnCA as a promising antibiotic substitute for the treatment of diarrhea in humans.
引起疾病的微生物，如大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus），是导致人类腹泻发病率和死亡率的主要因素之一。考虑到抗生素使用所带来的挑战，包括抗药性问题，本研究旨在开发一种新型的锌基制剂来灭活细菌。为此，使用己酸（CA）和氧化锌（ZnO）在无水乙醇中通过溶剂热法合成了己酸锌（ZnCA）。结构特征分析技术，包括傅里叶变换红外光谱、单晶 X 射线衍射分析和核磁共振波谱，揭示了锌原子与 CA 的双齿桥联配位。结果表明，二维 ZnCA 网络由独特的层状图案组成，没有明显的层间相互作用。粉末 X 射线衍射分析、元素分析和熔点分析证实，ZnCA 的平均粒径为 1.320 µm，熔点为 147.2°C，纯度超过 98%。值得注意的是，ZnCA 对大肠杆菌和金黄色葡萄球菌表现出很强的抗菌活性，超过了 ZnO 的抗菌效果。ZnCA 通过抑制生物膜形成、破坏细胞膜完整性、增加细胞膜通透性和改变细胞内 Ca-Mg-ATP 酶活性来发挥其抗菌作用。这些发现强调了 ZnCA 作为一种有前途的抗生素替代品，用于治疗人类腹泻的潜力。
