Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs of China, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China.
FEMS Microbiol Ecol. 2024 May 14;100(6). doi: 10.1093/femsec/fiae034.
The shelled pteropod Creseis acicula is a marine pelagic shellfish widely distributed from temperate to tropical seas around the world. From June to July 2020, a C. acicula bloom first happened in the Daya Bay, southern China, and its density reached the highest value (5600 ind. m-3) ever recorded around the world. However, few studies have investigated the responses of bacterial communities to the C. acicula bloom. In the present study, we examined the community profiles of three communities of bacteria including the free-living and particle-attached bacteria in the blooming and reference waters, and bacteria attached to the whole body and shell of C. acicula using a high-throughput sequencing method. The results indicated that the C. acicula bloom had a greater impact on particle-attached bacteria than free-living bacteria. Among the bloom-sensitive particle-attached bacteria, the predominant bacterial phyla were Pseudomonadota, Bacteroidota and Verrucomicrobiota in the blooming areas, whereas they were Actinomycetota and Planctomycetota in the reference areas. Specifically, fecal bacteria Haloferula and Halioglobus spp. were significantly enriched in the blooming waters and accumulated on C. acicula shells. Conversely, the significantly lower relative abundance of Nocardioides sp. in the blooming area and accumulated on the whole body of C. acicula indicated their attachment to particles consumed by C. acicula. Overall, our results suggested that the C. acicula bloom influenced marine bacteria, particularly particle-attached bacteria, by increasing (e.g. providing shells and feces) or decreasing (e.g. filter-feeding the suspended particles) the abundance of available substances.
有壳翼足类生物 C. acicula 是一种广泛分布于世界各地温带和热带海域的海洋浮游贝类。2020 年 6 月至 7 月，中国南部的大亚湾首次发生 C. acicula 水华，其密度达到了有记录以来的全球最高值（5600 个 ind. m-3）。然而，很少有研究调查细菌群落对 C. acicula 水华的响应。在本研究中，我们使用高通量测序方法检测了富营养化和对照水域中自由生活和颗粒附着细菌以及附着在 C. acicula 整个身体和贝壳上的细菌的群落组成。结果表明，C. acicula 水华对颗粒附着细菌的影响大于自由生活细菌。在富营养化敏感的颗粒附着细菌中，优势细菌门在富营养化区域是假单胞菌门、拟杆菌门和疣微菌门，而在对照区域是放线菌门和浮霉菌门。具体来说，粪便细菌 Haloferula 和 Halioglobus spp. 在富营养化水域中显著富集，并在 C. acicula 壳上积累。相反，在富营养化区域中 Nocardioides sp. 的相对丰度显著降低，并且在 C. acicula 全身积累，表明它们附着在 C. acicula 消耗的颗粒上。总的来说，我们的结果表明，C. acicula 水华通过增加（例如提供贝壳和粪便）或减少（例如滤食悬浮颗粒）可用物质的丰度来影响海洋细菌，特别是颗粒附着细菌。
