Zhao Zelong, Wang Xuda, Jiang Jingwei, Dong Ying, Pan Yongjia, Guan Xiaoyan, Wang Bai, Gao Shan, Chen Zhong, Zhou Zunchun
Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
Chemosphere. 2023 Jul;330:138568. doi: 10.1016/j.chemosphere.2023.138568. Epub 2023 Apr 3.
The mariculture environment is a sink of microplastics (MPs) due to its enclosed nature and mass use of plastics. Nanoplastics (NPs) are MPs with a diameter <1 μm that have a more toxic effect on aquatic organisms than other MPs. However, little is known about the underlying mechanisms of NP toxicity on mariculture species. Here, we performed a multi-omics investigation to explore gut microbiota dysbiosis and associated health problems induced by NPs in juvenile sea cucumber Apostichopus japonicus, a commercially and ecologically important marine invertebrate. We observed significant differences in gut microbiota composition after 21 days of NP exposure. Ingestion of NPs significantly increased core gut microbes, especially Rhodobacteraceae and Flavobacteriaceae families. Additionally, gut gene expression profiles were altered by NPs, especially those related to neurological diseases and movement disorders. Correlation and network analyses indicated close relationships between transcriptome changes and gut microbiota variation. Furthermore, NPs induced oxidative stress in sea cucumber intestines, which may be associated with intraspecies variation in Rhodobacteraceae in the gut microbiota. The results suggested that NPs were harmful to the health of sea cucumbers, and they highlighted the importance of the gut microbiota in the responses to NP toxicity in marine invertebrates.
由于海水养殖环境具有封闭性且大量使用塑料，它已成为微塑料的汇聚地。纳米塑料是直径小于1微米的微塑料，对水生生物的毒性比其他微塑料更强。然而，纳米塑料对海水养殖物种毒性作用的潜在机制仍知之甚少。在此，我们开展了一项多组学研究，以探究纳米塑料对重要商业和生态意义的海洋无脊椎动物——幼年刺参肠道微生物群失调及相关健康问题的影响。在纳米塑料暴露21天后，我们观察到肠道微生物群组成存在显著差异。摄入纳米塑料显著增加了核心肠道微生物，尤其是红杆菌科和黄杆菌科。此外，纳米塑料改变了肠道基因表达谱，尤其是与神经疾病和运动障碍相关的基因表达谱。相关性和网络分析表明转录组变化与肠道微生物群变异之间存在密切关系。此外，纳米塑料在刺参肠道中诱导了氧化应激，这可能与肠道微生物群中红杆菌科的种内变异有关。研究结果表明，纳米塑料对刺参健康有害，同时也凸显了肠道微生物群在海洋无脊椎动物应对纳米塑料毒性方面的重要性。
