Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
Unidade de Microbiologia, Serviço de Diagnóstico Laboratorial - Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
Diagn Microbiol Infect Dis. 2024 Jun;109(2):116271. doi: 10.1016/j.diagmicrobio.2024.116271. Epub 2024 Mar 19.
This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92.05 %. Regarding the rapid broth microdilution for Fluconazole and Micafungin, the agreement was 97.06 % (p<0.001) and 100 % (p<0.001), and the Kappa coefficient was 0.91 (p<0.001) and 1.0 (p<0.001), respectively. To conclude, both rapid methods showed to be reproducible, inexpensive, easy to perform and time-saving. Thus, these methodologies could be useful to guide and adjust empirical antifungal therapy.
本研究旨在开发和验证一种通过 MALDI-TOF 系统快速鉴定和肉汤微量稀释法测定血流感染相关念珠菌对氟康唑和米卡芬净敏感性的方法。从血培养瓶中进行的亚培养物孵育 5 小时（+/-1 小时），并用于进行检测，因此快速鉴定和药敏谱的周转时间分别约为 5 小时和 24 小时。快速鉴定的符合率为 92.05%。关于快速肉汤微量稀释法测定氟康唑和米卡芬净，符合率分别为 97.06%（p<0.001）和 100%（p<0.001），Kappa 系数分别为 0.91（p<0.001）和 1.0（p<0.001）。总之，这两种快速方法都具有可重复性、成本低、易于操作和节省时间的特点。因此，这些方法可能有助于指导和调整经验性抗真菌治疗。
