• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脑蛋白水解物和胞磷胆碱通过上调受影响神经细胞中脑源性神经营养因子（BDNF）的表达实现神经保护作用：一项体外研究获得的初步线索

Neuroprotection by Cerebrolysin and Citicoline Through the Upregulation of Brain-Derived Neurotrophic Factor (BDNF) Expression in the Affected Neural Cells: A Preliminary Clue Obtained Through an In Vitro Study.

作者信息

P Anandan, Rengarajan Santhanam, Venkatachalam Sankar, Pattabi Sasikumar, Jones Sumathi, K Prabhu, Krishna Vani, Prasanth Krishna

机构信息

Department of General Medicine, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai, IND.

Department of Neurosurgery, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai, IND.

出版信息

Cureus. 2024 Feb 21;16(2):e54665. doi: 10.7759/cureus.54665. eCollection 2024 Feb.

DOI:10.7759/cureus.54665
PMID:38524067
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10960614/
Abstract

OBJECTIVES

Citicoline and cerebrolysin are two unique yet contentious medications because of inconsistencies in efficacy as well as the mystery surrounding their mode of action. The current study aimed to re-validate the neuroprotective benefits of these medications and investigate the possible molecular mechanism.

METHODS

Neuro-2A cells were exposed to tert-butyl hydroperoxide, a consistent in vitro model of neuronal damage caused by oxidative stress. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, acridine orange/ethidium bromide (AO-EtBr) staining, and phase-view examinations were utilized to evaluate cell survival and cytotoxicity. Real-time reverse transcription-polymerase chain reaction (RT-PCR)-based gene expression studies were conducted.

KEY FINDING

Observations revealed that these two medications had modest but considerable neuroprotective effects. While the majority of the genes' expressions remained unchanged, cerebrolysin upregulated Neuregulin 1, and both upregulated brain-derived neurotrophic factor (BDNF) expression.

CONCLUSION

The findings of the current study may be the first to suggest that citicoline and cerebrolysin may increase host cells' defense mechanisms (secretion neurotrophic factors) rather than carrying nutrients for cell survival. Because of its simplicity, the current study can readily be repeated to learn more about these two disputed medications for treating ischemic stroke.

摘要

目的

胞磷胆碱和脑蛋白水解物是两种独特但存在争议的药物，原因在于其疗效不一致以及作用机制不明。本研究旨在重新验证这些药物的神经保护作用，并探究其可能的分子机制。

方法

将Neuro-2A细胞暴露于叔丁基过氧化氢，这是一种由氧化应激导致神经元损伤的体外模型。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、吖啶橙/溴化乙锭（AO-EtBr）染色及相差显微镜检查评估细胞存活和细胞毒性。进行基于实时逆转录-聚合酶链反应（RT-PCR）的基因表达研究。

主要发现

观察结果显示，这两种药物具有适度但显著的神经保护作用。虽然大多数基因的表达保持不变，但脑蛋白水解物上调了神经调节蛋白1，两者均上调了脑源性神经营养因子（BDNF）的表达。

结论

本研究结果可能首次表明，胞磷胆碱和脑蛋白水解物可能增强宿主细胞的防御机制（分泌神经营养因子），而非为细胞存活携带营养物质。鉴于本研究的简易性，可轻易重复进行以进一步了解这两种用于治疗缺血性中风的争议药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/923a02e7060a/cureus-0016-00000054665-i06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/14be3334d8c7/cureus-0016-00000054665-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/b5c0ecc198cb/cureus-0016-00000054665-i02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/7e1bb1ef1646/cureus-0016-00000054665-i03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/c66c71bdbf79/cureus-0016-00000054665-i04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/1b952d114b99/cureus-0016-00000054665-i05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/923a02e7060a/cureus-0016-00000054665-i06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/14be3334d8c7/cureus-0016-00000054665-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/b5c0ecc198cb/cureus-0016-00000054665-i02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/7e1bb1ef1646/cureus-0016-00000054665-i03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/c66c71bdbf79/cureus-0016-00000054665-i04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/1b952d114b99/cureus-0016-00000054665-i05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c76/10960614/923a02e7060a/cureus-0016-00000054665-i06.jpg

相似文献

1
Neuroprotection by Cerebrolysin and Citicoline Through the Upregulation of Brain-Derived Neurotrophic Factor (BDNF) Expression in the Affected Neural Cells: A Preliminary Clue Obtained Through an In Vitro Study.脑蛋白水解物和胞磷胆碱通过上调受影响神经细胞中脑源性神经营养因子（BDNF）的表达实现神经保护作用：一项体外研究获得的初步线索
Cureus. 2024 Feb 21;16(2):e54665. doi: 10.7759/cureus.54665. eCollection 2024 Feb.
2
Superior neuroprotective effects of cerebrolysin in heat stroke following chronic intoxication of Cu or Ag engineered nanoparticles. A comparative study with other neuroprotective agents using biochemical and morphological approaches in the rat.脑蛋白水解物对铜或银工程纳米颗粒慢性中毒后中暑的卓越神经保护作用。在大鼠中使用生化和形态学方法与其他神经保护剂的比较研究。
J Nanosci Nanotechnol. 2011 Sep;11(9):7549-69. doi: 10.1166/jnn.2011.5114.
3
Citicoline/Coenzyme Q10/Vitamin B3 Fixed Combination Exerts Synergistic Protective Effects on Neuronal Cells Exposed to Oxidative Stress.胞磷胆碱/辅酶 Q10/烟酰胺三联复合物对氧化应激状态下神经元细胞发挥协同保护作用。
Nutrients. 2022 Jul 20;14(14):2963. doi: 10.3390/nu14142963.
4
Cerebrolysin protects PC12 cells from CoCl2-induced hypoxia employing GSK3β signaling.脑蛋白水解物通过糖原合成酶激酶3β信号通路保护PC12细胞免受氯化钴诱导的缺氧损伤。
Int J Dev Neurosci. 2014 Nov;38:52-8. doi: 10.1016/j.ijdevneu.2014.07.005. Epub 2014 Aug 2.
5
Efficacy of Neuroprotective Drugs in Acute Ischemic Stroke: Is It Helpful?神经保护药物在急性缺血性卒中中的疗效：是否有益？
J Neurosci Rural Pract. 2019 Oct;10(4):576-581. doi: 10.1055/s-0039-1700790. Epub 2019 Dec 11.
6
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.脑活素对毛果芸香碱诱导癫痫发作后海马神经元死亡的影响。
Front Neurosci. 2020 Oct 16;14:568813. doi: 10.3389/fnins.2020.568813. eCollection 2020.
7
Cerebrolysin, a mixture of neurotrophic factors induces marked neuroprotection in spinal cord injury following intoxication of engineered nanoparticles from metals.脑活素，一种神经营养因子混合物，在金属工程纳米粒子中毒导致的脊髓损伤后能显著诱导神经保护。
CNS Neurol Disord Drug Targets. 2012 Feb;11(1):40-9. doi: 10.2174/187152712799960781.
8
Improvement in functional recovery with administration of Cerebrolysin after experimental closed head injury.脑活素治疗实验性闭合性颅脑损伤后功能恢复的改善。
J Neurosurg. 2013 Jun;118(6):1343-55. doi: 10.3171/2013.3.JNS122061. Epub 2013 Apr 12.
9
Cerebrolysin reduces blood-cerebrospinal fluid barrier permeability change, brain pathology, and functional deficits following traumatic brain injury in the rat.脑活素可降低创伤性脑损伤大鼠血脑屏障通透性改变、脑病理和功能缺陷。
Ann N Y Acad Sci. 2010 Jun;1199:125-37. doi: 10.1111/j.1749-6632.2009.05329.x.
10
Concussive head injury exacerbates neuropathology of sleep deprivation: Superior neuroprotection by co-administration of TiO-nanowired cerebrolysin, alpha-melanocyte-stimulating hormone, and mesenchymal stem cells.脑震荡性头部损伤会加剧睡眠剥夺的神经病理学：联合使用二氧化钛纳米线脑蛋白水解物、α-黑素细胞刺激素和间充质干细胞具有更好的神经保护作用。
Prog Brain Res. 2020;258:1-77. doi: 10.1016/bs.pbr.2020.09.003. Epub 2020 Nov 2.

引用本文的文献

1
Current neuroprotective agents in stroke.当前用于中风的神经保护剂。
Turk J Phys Med Rehabil. 2024 May 16;70(2):157-163. doi: 10.5606/tftrd.2024.15287. eCollection 2024 Jun.

本文引用的文献

1
Cerebrolysin for acute ischaemic stroke.脑蛋白水解物用于急性缺血性卒中
Cochrane Database Syst Rev. 2020 Jul 14;7(7):CD007026. doi: 10.1002/14651858.CD007026.pub6.
2
Hope Dies Last-Evidence Again Fails to Support a Neuroprotectant: Cerebrolysin for Acute Ischemic Stroke.希望最后破灭——证据再次无法支持一种神经保护剂：脑蛋白水解物治疗急性缺血性卒中
Stroke. 2017 Sep;48(9):2343-2344. doi: 10.1161/STROKEAHA.117.018202. Epub 2017 Jul 26.
3
Efficacy and Safety of Cerebrolysin for Acute Ischemic Stroke: A Meta-Analysis of Randomized Controlled Trials.
脑蛋白水解物治疗急性缺血性脑卒中的疗效与安全性：一项随机对照试验的Meta分析
Biomed Res Int. 2017;2017:4191670. doi: 10.1155/2017/4191670. Epub 2017 Jun 5.
4
Is aura around citicoline fading? A systemic review.胞磷胆碱周围光环正在消退吗？系统评价。
Indian J Pharmacol. 2017 Jan-Feb;49(1):4-9. doi: 10.4103/0253-7613.201037.
5
JNK1 and JNK3 play a significant role in both neuronal apoptosis and necrosis. Evaluation based on in vitro approach using tert-butylhydroperoxide induced oxidative stress in neuro-2A cells and perturbation through 3-aminobenzamide.JNK1和JNK3在神经元凋亡和坏死过程中均发挥重要作用。基于体外实验方法进行评估，该方法利用叔丁基过氧化氢在Neuro-2A细胞中诱导氧化应激，并通过3-氨基苯甲酰胺进行干扰。
Toxicol In Vitro. 2017 Jun;41:168-178. doi: 10.1016/j.tiv.2017.02.015. Epub 2017 Feb 28.
6
The logistics of myelin biogenesis in the central nervous system.中枢神经系统中髓鞘形成的物流学。
Glia. 2017 Jul;65(7):1021-1031. doi: 10.1002/glia.23116. Epub 2017 Feb 7.
7
Cerebrolysin combined with rehabilitation promotes motor recovery in patients with severe motor impairment after stroke.脑蛋白水解物联合康复治疗可促进脑卒中后严重运动功能障碍患者的运动功能恢复。
BMC Neurol. 2016 Mar 2;16:31. doi: 10.1186/s12883-016-0553-z.
8
Cerebrolysin dose-dependently improves neurological outcome in rats after acute stroke: A prospective, randomized, blinded, and placebo-controlled study.脑活素在急性中风大鼠中剂量依赖性地改善神经功能结局：一项前瞻性、随机、双盲、安慰剂对照研究。
Int J Stroke. 2016 Apr;11(3):347-55. doi: 10.1177/1747493015625645. Epub 2016 Jan 12.
9
Cerebrolysin and Recovery After Stroke (CARS): A Randomized, Placebo-Controlled, Double-Blind, Multicenter Trial.脑活素与卒中后恢复（CARS）：一项随机、安慰剂对照、双盲、多中心试验。
Stroke. 2016 Jan;47(1):151-9. doi: 10.1161/STROKEAHA.115.009416. Epub 2015 Nov 12.
10
Acute stroke intervention: a systematic review.急性脑卒中干预：系统评价。
JAMA. 2015 Apr 14;313(14):1451-62. doi: 10.1001/jama.2015.3058.