He Minghui, Han Yanlong, Gao Yong, Han Min, Duan Liqing
College of Forestry, Inner Mongolia Agricultural University, Hohhot, China.
College of Desert Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.
Front Plant Sci. 2024 Feb 26;15:1339424. doi: 10.3389/fpls.2024.1339424. eCollection 2024.
The population of , situated on the edge of the typical grassland-to-desert transition in the Mu Us Sandy Land, plays a vital ecological role in maintaining stability within the regional fragile ecosystem. Despite the consistent growth of following animal grazing, the biological mechanisms underlying its compensatory growth in response to livestock consumption remain unclear. Analyzing 48 metabolomic profiles from , our study reveals that the grazing process induces significant changes in the metabolic pathways of branches. Differential metabolites show correlations with soluble protein content, catalase, peroxidase, superoxide dismutase, malondialdehyde, and proline levels. Moreover, machine learning models built on these differential metabolites accurately predict the intensity of grazing (with an accuracy of 83.3%). The content of various metabolites, indicative of plant stress responses, including Enterolactone, Narceine, and Folcepri, exhibits significant variations in response to varying grazing intensities (0.05). Our investigation reveals that elevated grazing intensity intensifies the stress response in , triggering heightened antioxidative defenses and stress-induced biochemical activities. Distinctive metabolites play a pivotal role in responding to stress, facilitating the plant's adaptation to environmental challenges and fostering regeneration.
位于毛乌素沙地典型草原向沙漠过渡边缘的[物种名称]，在维持区域脆弱生态系统的稳定性方面发挥着至关重要的生态作用。尽管[物种名称]在动物放牧后持续生长，但其对牲畜啃食产生补偿性生长的生物学机制仍不清楚。通过分析来自[物种名称]的48个代谢组学图谱，我们的研究表明放牧过程会引起[物种名称]枝条代谢途径的显著变化。差异代谢物与可溶性蛋白含量、过氧化氢酶、过氧化物酶、超氧化物歧化酶、丙二醛和脯氨酸水平存在相关性。此外，基于这些差异代谢物构建的机器学习模型能够准确预测[物种名称]的放牧强度（准确率为83.3%）。包括肠内酯、那可丁和福西普里在内的各种指示植物应激反应的代谢物含量，在不同放牧强度下呈现出显著差异（P<0.05）。我们的研究表明，放牧强度的增加会加剧[物种名称]的应激反应，引发更强的抗氧化防御和应激诱导的生化活动。独特的代谢物在应对压力方面起着关键作用，有助于植物适应环境挑战并促进再生。
