Khalilian Parvin, Eskandari Nahid, Sharifi Mohammad Jafar, Soltani Mohammad, Nematollahi Pardis
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Applied Physiology Research Center, Isfahan Cardiovascular Research Institute, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2024 Feb 26;13:17. doi: 10.4103/abr.abr_67_23. eCollection 2024.
Myelodysplastic syndrome (MDS) is a clonal hematologic disorder that requires the integration of morphologic, cytogenetic, hematologic, and clinical findings for a successful diagnosis. Trying to find ancillary tests such as biomarkers improve the diagnosis process. Several studies showed that a disordered immune system is associated with MDS. The chronic activated innate immune system, particularly the Toll-like receptors (TLRs) pathway could be involved in the induction of the inflammation.
In the present study, we investigated the expression of , and in bone marrow (BM) of MDS patients, the leukemia group, and the healthy group. For this purpose, we assessed the expression of , and by real time-PCR.
In line with new findings, we demonstrated that the expression of , and significantly increased in MDS BM compared with the healthy group. Moreover, expression raised significantly in MDS patients compared with other studied hematologic neoplasms. Also, the expression levels of and significantly increased in MDS in comparison to some studied non-MDS malignancies ( ˂ 0.05). Receiver operating characteristics (ROC) analysis and area under the curve (AUC) suggested that the expression of , and (AUC = 0.702, AUC = 0.75, and AUC = 0.682, respectively) had acceptable diagnostic values to identify MDS from the other understudied leukemias.
Overall, the expression of , and could be potential biomarkers for discriminating MDS from some hematologic disorders.
骨髓增生异常综合征（MDS）是一种克隆性血液系统疾病，需要整合形态学、细胞遗传学、血液学和临床检查结果才能成功诊断。尝试寻找生物标志物等辅助检查可改善诊断过程。多项研究表明，免疫系统紊乱与MDS有关。慢性激活的固有免疫系统，尤其是Toll样受体（TLR）通路可能参与炎症的诱导。
在本研究中，我们调查了MDS患者、白血病组和健康组骨髓中[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达情况。为此，我们通过实时定量聚合酶链反应评估了[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达。
与新发现一致，我们证明与健康组相比，MDS骨髓中[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达显著增加。此外，与其他研究的血液系统肿瘤相比，MDS患者中[具体基因名称未给出]的表达显著升高。而且，与一些研究的非MDS恶性肿瘤相比，MDS中[具体基因名称未给出]和[具体基因名称未给出]的表达水平也显著增加（P˂0.05）。受试者工作特征（ROC）分析和曲线下面积（AUC）表明，[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达（AUC分别为0.702、0.则可从其他研究较少的白血病中鉴别出MDS。
总体而言，[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的表达可能是区分MDS与某些血液系统疾病的潜在生物标志物。 75和0.682）具有可接受的诊断价值，以识别MDS。
