Department of Anatomy, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Anat Histol Embryol. 2024 May;53(3):e13032. doi: 10.1111/ahe.13032.
The objective of this study is to assess the efficacy of a solution including honey, ethyl alcohol, liquid paraffin, distilled water and citric acid (HEFS) as a preservative for rabbit cadavers, serving as a potential substitute for formaldehyde. The cadavers underwent preservation using three distinct solutions: 10% formalin, 35% alcohol and HEFS. The cadavers were subjected to a total of four sampling events, occurring at 4-month intervals, in order to collect specimens for microanatomical, histological, microbiological, mycological, colourimetric, texture and odour analysis. In terms of hardness, suitability for dissection and joint mobility metrics, the cadavers fixed with HEFS had superior qualities to those fixed with formalin. The fixation quality of HEFS for histological analyses was deemed acceptable, except kidney and intestinal tissues. In texture analysis, differences only in the elasticity parameter (p < 0.05) in the same sampling period. A total of 10 (13.9) bacteria isolates were identified among which, Metasolibacillus meyeri 3 (30%) was predominantly followed by Staphylococcus aureus 2 (20%), Bacillus siamensis, Bacillus subtilis, Pseudarthrobacter oxydans, Bacillus licheniformis, Bacillus subtilis subsp. subtilis with a proportion of 1 (10%), respectively, by both microbiological and molecular analysis. However, no anaerobic bacteria and fungi were isolated. A considerable percentage of the students had the perception that HEFS was appropriate for utilization in laboratory settings due to its absence of unpleasant odours and detrimental impact on ocular and respiratory functions. In conclusion, we consider that HEFS may serve as a viable substitute for formalin solution in the preservation of rabbit cadavers.
本研究旨在评估一种包括蜂蜜、乙醇、液体石蜡、蒸馏水和柠檬酸（HEFS）的溶液作为甲醛替代物保存兔尸体的功效。将尸体分别使用三种不同的溶液（10%福尔马林、35%酒精和 HEFS）进行保存。共进行了四次取样，每四个月进行一次，以收集微观解剖学、组织学、微生物学、真菌学、比色、质地和气味分析的标本。就硬度、解剖适用性和关节活动性指标而言，用 HEFS 固定的尸体比用甲醛固定的尸体质量更好。HEFS 固定用于组织学分析的质量是可以接受的，但肾脏和肠道组织除外。在质地分析中，仅在同一采样期内的弹性参数上存在差异（p<0.05）。在总共 10（13.9）个细菌分离株中，梅氏乳杆菌 3（30%）占主导地位，其次是金黄色葡萄球菌 2（20%）、暹罗芽孢杆菌、枯草芽孢杆菌、氧化假单胞菌、地衣芽孢杆菌、枯草芽孢杆菌亚种。枯草芽孢杆菌subsp。subtilis 分别为 1（10%），这两种微生物和分子分析方法都得到了证实。然而，没有分离出厌氧菌和真菌。相当一部分学生认为 HEFS 由于没有难闻的气味，并且对眼睛和呼吸系统功能没有不良影响，因此适合在实验室环境中使用。总之，我们认为 HEFS 可以作为福尔马林溶液保存兔尸体的一种可行替代品。
