Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Department of Anatomy and Histology, Faculty of Veterinary Medicine, King Salman International University, Ras Sudr, South Saini, Egypt.
Anat Histol Embryol. 2024 May;53(3):e13036. doi: 10.1111/ahe.13036.
The present study focused on the histogenesis of the crop in quail embryos. The developmental sequence was recorded from day 10 of incubation to the hatching day (17th day) by gross anatomy, morphometric analysis and by using light and scanning electron microscope (SEM). Grossly, the crop was distinguished as an enlarged pouch of the cervical oesophagus on the 12th day of incubation. Histologically, the development of the crop began on the 10th day of incubation. The muscularis mucosae was observed as a small, thin layer of myoblast cells on the 10th day, then it appeared as longitudinally arranged smooth muscle fibres with progression of the developmental age till hatching day. The submucosal layer was also observed on the 10th day, while the lamina propria, muscular layer (inner circular and outer longitudinal layers of smooth muscle fibre) and glandular primordia were detected on the 12th day. The epithelial cells of crop revealed affinity for PAS stain and AB-PAS on the 10th day of incubation till the 17th day, while the affinity for AB stain appeared on the 14th day of incubation. Additionally, the glandular cells showed the positive for AB, PAS and AB-PAS on the 12th day of incubation. SEM sections showed variable sizes of the openings of the oesophageal glands. In conclusion, the present study provided a detailed overview of the histogenesis of the crop in quail embryos.
本研究专注于鹌鹑胚胎嗉囊的组织发生。通过大体解剖、形态计量分析以及使用光镜和扫描电子显微镜（SEM），从孵化第 10 天到孵化日（第 17 天）记录了发育序列。大体上，嗉囊在孵化第 12 天被区分出来，是颈食管的一个扩大囊。组织学上，嗉囊的发育始于孵化第 10 天。在孵化第 10 天，观察到黏膜肌层是一层小而薄的成肌细胞。然后，随着发育年龄的进展，它呈现出纵向排列的平滑肌纤维，直到孵化日。在孵化第 10 天也观察到黏膜下层，而固有层、肌层（内环和外纵平滑肌纤维层）和腺原基则在孵化第 12 天被检测到。嗉囊的上皮细胞在孵化第 10 天到第 17 天都对 PAS 染色和 AB-PAS 染色具有亲和力，而对 AB 染色的亲和力则在孵化第 14 天出现。此外，在孵化第 12 天，腺细胞对 AB、PAS 和 AB-PAS 呈阳性。SEM 切片显示食管腺开口的大小不同。总之，本研究提供了鹌鹑胚胎嗉囊组织发生的详细概述。
