Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
Department of Stem Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Microsc Res Tech. 2022 Jan;85(1):156-168. doi: 10.1002/jemt.23892. Epub 2021 Aug 3.
To understand the development of the mucous preglottal salivary gland in Coturnix japonica (Japanese quail), morphological and histochemical studies were performed on 20 healthy Japanese quail embryos (aging from 10th to 17th incubation days) and 25 healthy quail chicks (aging from 0th to 60th days). The primordia of preglottal salivary gland were observed as an epithelial bud at the early embryonic stage, which then elongated and differentiated into secretory units by the end of this stage. In Japanese quails, the preglottal salivary gland was a mucous polystomatic tubuloalveolar unpaired gland composed of two lateral portions and a middle one embedded into the submucosa of the lingual root. The gland openings accompanied taste pore (8.17 μm) of taste buds associated salivary glands type; some skeletal muscle fibers embedded among secretory lobules extended from muscle cricohyoideus at 14th day-old quail chick. Also, both herbts corpuscles and secretory motor plexus could be detected among secretory lobules. Based on our investigations, the development of the preglottal salivary gland could clearly be distinguished in the embryonic stage into pre bud and bud stages at 10th day old, cord and branching stages ended by cavitation at 11th day old, canalization stage at 13th day old, lobulation and secretory stages by the 17th day old. The secretory materials showed different histochemical reactions ended with highly alcinophilic mucous indicated highly sialomucin (acidic) content. Myoepithelial cells could be demonstrated at a 17-day old quail embryo and thereafter surrounded the secretory endpieces of the preglottal salivary gland.
为了了解日本鹌鹑（ Coturnix japonica ）的会厌前黏液腺的发育情况，对 20 只健康的日本鹌鹑胚胎（孵化第 10 至 17 天）和 25 只健康的鹌鹑雏鸡（孵化第 0 至 60 天）进行了形态学和组织化学研究。在早期胚胎阶段，会厌前唾液腺的原基被观察为一个上皮芽，然后在这个阶段结束时伸长并分化为分泌单位。在日本鹌鹑中，会厌前唾液腺是一种黏液多形管泡型单对腺体，由两个侧部和一个嵌入舌根黏膜下的中部组成。腺口伴随着味觉孔（8.17μm），属于与味觉腺相关的唾液腺类型；一些嵌入分泌小叶之间的骨骼肌纤维从 14 日龄鹌鹑雏鸡的环状舌骨肌延伸而来。此外，在分泌小叶中还可以检测到贺氏小体和分泌运动丛。根据我们的研究，在胚胎阶段，会厌前唾液腺的发育可以清楚地分为第 10 天的前芽和芽期、第 11 天结束的索状和分支期、第 13 天的管化期、第 17 天的小叶形成和分泌期。分泌物质显示出不同的组织化学反应，以高度嗜碱性黏液表示高度唾液黏蛋白（酸性）含量。17 日龄鹌鹑胚胎中可以显示出肌上皮细胞，此后它们包围会厌前唾液腺的分泌末端。
