Duke Human Vaccine Institute, Department of Medicine, School of Medicine, Duke University, Durham, North Carolina.
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
J Infect Dis. 2024 Oct 16;230(4):852-856. doi: 10.1093/infdis/jiae153.
There is an urgent need for vaccines against Neisseria gonorrhoeae, the causative agent of gonorrhea. Vaccination with an outer membrane vesicle-based Neisseria meningitidis vaccine provides some protection from N. gonorrhoeae; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against N gonorrhoeae antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and interassay variability, and robustness to sample storage conditions. The assay was then used to test samples from mice and humans immunized with an N meningitidis outer membrane vesicle vaccine.
目前迫切需要针对淋病奈瑟菌（导致淋病的病原体）的疫苗。接种脑膜炎奈瑟菌外膜囊泡疫苗可提供针对淋病奈瑟菌的一定程度的保护；然而，这种交叉保护的机制尚不清楚。为满足这一需求，我们开发了基于多重珠粒的分析方法，用于相对定量针对淋病奈瑟菌抗原的人源和鼠源 IgG 和 IgA。评估了分析方法的分析物独立性、稀释线性、特异性、灵敏度、批内和批间变异性以及对样品储存条件的稳健性。然后，使用该方法检测了接种脑膜炎奈瑟菌外膜囊泡疫苗的小鼠和人类的样本。
