Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
Methods Mol Biol. 2024;2793:3-19. doi: 10.1007/978-1-0716-3798-2_1.
Phage display is an important technology to study protein-protein interaction and protein evolution, with applications in basic science and applied biotechnology, such as drug discovery and the development of targeted therapies. However, in order to be successful during a phage display screening, it is paramount to have good phage libraries. Here, we described detailed procedures to generate peptide phage display libraries with high diversity and billions of transformants.
噬菌体展示是研究蛋白质-蛋白质相互作用和蛋白质进化的重要技术，在基础科学和应用生物技术方面有广泛应用，如药物发现和靶向治疗的发展。然而，为了在噬菌体展示筛选中取得成功，拥有良好的噬菌体文库是至关重要的。在这里，我们描述了生成具有高多样性和数十亿转化体的肽噬菌体展示文库的详细步骤。
