Smith G P
Science. 1985 Jun 14;228(4705):1315-7. doi: 10.1126/science.4001944.
Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.
外源DNA片段可插入丝状噬菌体基因III，以产生一种中间带有外源序列的融合蛋白。该融合蛋白被整合到病毒粒子中，病毒粒子保留感染性，并以免疫可及的形式展示外源氨基酸。通过与针对外源序列的抗体的亲和力，这些“融合噬菌体”可比普通噬菌体富集1000倍以上。当有针对该基因产物的抗体时，融合噬菌体可为克隆基因提供一种简单的方法。
