Zhuang Jingshen, Chen Qianling, Xu Luyao, Qiao Dongfang, Chen Xuebing
Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, P. R. China.
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, P. R. China.
Electrophoresis. 2024 May;45(9-10):958-969. doi: 10.1002/elps.202300190. Epub 2024 Mar 25.
Methamphetamine (MA) is a highly addictive mental stimulant, and MA abuse remains a significant public health problem worldwide, while effective treatment options are limited. Lycium barbarum polysaccharide (LBP), a major effective component extracted from Lycium barbarum, has potential health-promoting effects on the nervous system; however, its role in MA dependence remains unclear. In this study, the conditioned place preference (CPP) of MA addiction in adult male mice was established to detect changes in gut microbiota profiles after LBP treatment through 16S rRNA gene sequencing. Our results found that LBP administration could alleviate MA-induced CPP and hyperactivity. Interestingly, LBP improved MA-induced gut microbiota dysbiosis by increasing some beneficial autochthonous genus abundances, such as Allobaculum, Gordonibacter, and Ileibacterium. MA exposure induced the co-occurrence network of intestinal microbiota to become weaker and more unstable when compared with the control group, while LBP changed the above effects when compared with the MA group. Bacterial gene function prediction showed that amphetamine addiction, cocaine addiction, and short-chain fatty acid metabolism were enriched. These findings reveal that LBP might regulate MA-induced gut microbiota and behavior changes, which showed potential therapeutic applicability in treating MA addiction by regulating the gut microbiota.
甲基苯丙胺（MA）是一种极易成瘾的精神兴奋剂，MA滥用在全球范围内仍然是一个重大的公共卫生问题，而有效的治疗选择却很有限。枸杞多糖（LBP）是从枸杞中提取的一种主要有效成分，对神经系统具有潜在的健康促进作用；然而，其在MA成瘾中的作用仍不清楚。在本研究中，通过16S rRNA基因测序建立成年雄性小鼠MA成瘾的条件性位置偏爱（CPP），以检测LBP治疗后肠道微生物群谱的变化。我们的结果发现，给予LBP可减轻MA诱导的CPP和多动。有趣的是，LBP通过增加一些有益的本土菌属丰度，如别氏菌属、戈登氏菌属和回肠杆菌属，改善了MA诱导的肠道微生物群失调。与对照组相比，MA暴露导致肠道微生物群的共现网络变得更弱且更不稳定，而与MA组相比，LBP改变了上述效应。细菌基因功能预测显示，苯丙胺成瘾、可卡因成瘾和短链脂肪酸代谢得到富集。这些发现揭示，LBP可能调节MA诱导的肠道微生物群和行为变化，这表明其在通过调节肠道微生物群治疗MA成瘾方面具有潜在的治疗适用性。
