Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2# Xuelin Road, Nanjing 210023, P. R. China.
J Agric Food Chem. 2024 Apr 10;72(14):8027-8038. doi: 10.1021/acs.jafc.4c00923. Epub 2024 Mar 26.
There is considerable research evidence that α-dicarbonyl compounds, including glyoxal (GO) and methylglyoxal (MGO), are closely related to many chronic diseases. In this work, after comparison of the capture capacity, reaction pathway, and reaction rate of synephrine (SYN) and neohesperidin (NEO) on GO/MGO , experimental mice were administrated with SYN and NEO alone and in combination. Quantitative data from UHPLC-QQQ-MS/MS revealed that SYN/NEO/HES (hesperetin, the metabolite of NEO) could form the GO/MGO-adducts in mice (except SYN-MGO), and the levels of GO/MGO-adducts in mouse urine and fecal samples were dose-dependent. Moreover, SYN and NEO had a synergistic scavenging effect on GO by promoting each other to form more GO adducts, while SYN could promote NEO to form more MGO-adducts, although it could not form MGO-adducts. Additionally, human experiments showed that the GO/MGO-adducts of SYN/NEO/HES found in mice were also detected in human urine and fecal samples after drinking flowers of L. var. Engl. (FCAVA) tea using UHPLC-QTOF-MS/MS. These findings provide a novel strategy to reduce endogenous GO/MGO via the consumption of dietary FCAVA rich in SYN and NEO.
有相当多的研究证据表明，α-二羰基化合物，包括乙二醛（GO）和甲基乙二醛（MGO），与许多慢性疾病密切相关。在这项工作中，在比较了辛弗林（SYN）和新橙皮苷（NEO）对 GO/MGO 的捕获能力、反应途径和反应速率后，单独和联合给实验小鼠施用了 SYN 和 NEO。来自 UHPLC-QQQ-MS/MS 的定量数据显示，SYN/NEO/HES（橙皮苷，NEO 的代谢物）可以在小鼠中形成 GO/MGO 加合物（除了 SYN-MGO），并且小鼠尿液和粪便样本中的 GO/MGO 加合物水平呈剂量依赖性。此外，SYN 和 NEO 对 GO 具有协同清除作用，通过促进彼此形成更多的 GO 加合物，而 SYN 可以促进 NEO 形成更多的 MGO 加合物，尽管它不能形成 MGO 加合物。此外，人体实验表明，在饮用富含 SYN 和 NEO 的 L. var. Engl.（FCAVA）茶后，在小鼠中发现的 SYN/NEO/HES 的 GO/MGO 加合物也在人体尿液和粪便样本中被检测到。这些发现为通过食用富含 SYN 和 NEO 的 FCAVA 来减少内源性 GO/MGO 提供了一种新策略。
