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Isolation of plasmid-protein complexes from Escherichia coli.

作者信息

Busby S, Kolb A, Buc H

出版信息

Eur J Biochem. 1979 Aug 15;99(1):105-11. doi: 10.1111/j.1432-1033.1979.tb13237.x.

DOI:10.1111/j.1432-1033.1979.tb13237.x
PMID:385318
Abstract

A procedure is described for the isolation of complexes between pMB9 plasmids and protein from Escherichia coli which are stable during centrifugation on sucrose gradients and are not destroyed in the presence of competitor DNA. The proteins in these complexes have been analysed by dodecyl sulphate/polyacrylamide gel electrophoresis. Only 10 polypeptide species are found in significant quantities, many of which are bound to both the plasmid and host DNA. We have also detected the presence of one protein which binds to a specific DNA sequence inserted in the plasmid.

摘要

相似文献

1
Isolation of plasmid-protein complexes from Escherichia coli.
Eur J Biochem. 1979 Aug 15;99(1):105-11. doi: 10.1111/j.1432-1033.1979.tb13237.x.
2
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[Proteins firmly bound to DNA in the E. coli nucleoid].[紧密结合于大肠杆菌类核体中DNA的蛋白质]
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引用本文的文献

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DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes.DNA采样：一种探测细菌染色体上特定位点蛋白质结合情况的方法。
Nucleic Acids Res. 2009 Apr;37(5):e37. doi: 10.1093/nar/gkp043. Epub 2009 Jan 30.
2
The recF-dependent endonuclease from Escherichia coli K12. Formation and resolution of pBR322 DNA multimers.来自大肠杆菌K12的RecF依赖性核酸内切酶。pBR322 DNA多聚体的形成与解析。
Mol Gen Genet. 1984;196(1):105-9. doi: 10.1007/BF00334100.
3
H1a, an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro.
H1a是一种在稳定期积累的大肠杆菌DNA结合蛋白，它在体外能使DNA强烈压缩。
Nucleic Acids Res. 1984 Jul 11;12(13):5321-40. doi: 10.1093/nar/12.13.5321.
4
Histonelike proteins of bacteria.细菌的组蛋白样蛋白
Microbiol Rev. 1987 Sep;51(3):301-19. doi: 10.1128/mr.51.3.301-319.1987.
5
Bacteriophage T4 late transcription from plasmid templates is enhanced by negative supercoiling.噬菌体T4从质粒模板的晚期转录通过负超螺旋得到增强。
J Bacteriol. 1988 Mar;170(3):1279-89. doi: 10.1128/jb.170.3.1279-1289.1988.