大肠杆菌K-12中共存质粒的拷贝数
Copy numbers of coexisting plasmids in Escherichia coli K-12.
作者信息
Barth P T, Richards H, Datta N
出版信息
J Bacteriol. 1978 Sep;135(3):760-5. doi: 10.1128/jb.135.3.760-765.1978.
Abstract
A strain of Escherichia coli K-12 carrying eight compatible and distinguishable plasmids was constructed. The amounts of plasmid DNA (measured as supercoiled molecules) per chromosome in this strain was about equal to the sum of the plasmid DNAs, extracted under controlled conditions, from strains each carrying one of the eight plasmids. Analysis of these DNA preparations showed that each plasmid in the multiplasmid strain was present in the same proportion per chromosome as in the single-plasmid strains. Also the level of phenotypic expression of each plasmid in the multiplasmid strain was the same as in the single-plasmid strains. Each plasmid, therefore, appears to control its own copy number irrespective of the presence of other compatible plasmids.
摘要
构建了一株携带八个相容且可区分质粒的大肠杆菌K - 12菌株。该菌株中每条染色体上的质粒DNA量（以超螺旋分子衡量）约等于在受控条件下从分别携带八个质粒之一的菌株中提取的质粒DNA总和。对这些DNA制剂的分析表明，多质粒菌株中每条染色体上每个质粒的比例与单质粒菌株相同。而且多质粒菌株中每个质粒的表型表达水平与单质粒菌株相同。因此，每个质粒似乎都能控制自身的拷贝数，而与其他相容质粒的存在无关。
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