Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Rua Monteiro Lobato, 80, Cidade Universitária Zeferino Vaz, Campinas, SP, CEP: 13083-862, Brazil.
Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Braz J Microbiol. 2024 Jun;55(2):1753-1758. doi: 10.1007/s42770-024-01316-y. Epub 2024 Mar 27.
Microbial pigments are considered as one of the main sources of natural types, and the attention to them is increasing in the food and pharmaceutical industries. This study aimed to investigate the effects of pigments extracted from Micrococcus roseus (PEM) on the gene expression of a and b staphylococcal enterotoxins (sea and seb) and their acute toxicity. Real-time PCR was used to study the anti-enterotoxigenic activity of PEM against Staphylococcus aureus at sub-inhibitory concentrations. In addition, the acute toxicity of PEM was evaluated on albino mice through alkaline phosphatase (ALP), aspartate aminotransferas (AST), and alanine aminotransferase (ALT) of liver and its histopathological changes. Based on the results, the expression of sea and seb was decreased in the presence of PEM at sub-inhibitory concentrations. The 2 was measured 0.02 and 0.01 for the expression of sea and seb of S. aureus grown in the MHB containing 16 mg/ml PEM. The results showed that the expression of seb is more sensitive to PEM compared to the expression of sea. After treatment of mice with PEM for two weeks, the condition of mice was normal, and the results of liver enzymatic activities and histopathological changes showed insignificant difference compared to the control sample.
微生物色素被认为是天然类型的主要来源之一，它们在食品和制药行业受到越来越多的关注。本研究旨在研究从玫瑰微球菌（PEM）中提取的色素对 a 和 b 葡萄球菌肠毒素（sea 和 seb）基因表达的影响及其急性毒性。实时 PCR 用于研究亚抑菌浓度下 PEM 对金黄色葡萄球菌的抗肠毒素活性。此外，通过碱性磷酸酶（ALP）、天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）评估 PEM 对肝脏及其组织病理学变化的急性毒性。根据结果，在亚抑菌浓度下存在 PEM 时，sea 和 seb 的表达减少。当 MHB 中含有 16mg/ml PEM 时，sea 和 seb 的表达分别为 0.02 和 0.01。结果表明，seb 的表达比 sea 对 PEM 更敏感。用 PEM 处理小鼠两周后，小鼠状况正常，与对照样品相比，肝酶活性和组织病理学变化的结果无显着差异。
