Gali Krishna Kishore, Jha Ambuj, Tar'an Bunyamain, Burstin Judith, Aubert Gregoire, Bing Dengjin, Arganosa Gene, Warkentin Thomas D
Department of Plant Sciences, Crop Development Centre, University of Saskatchewan, Saskatoon, SK, Canada.
School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India.
Front Plant Sci. 2024 Mar 11;15:1359117. doi: 10.3389/fpls.2024.1359117. eCollection 2024.
Improving the seed protein concentration (SPC) of pea ( L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries. To support the marker-assisted selection (MAS) of SPC towards accelerated breeding of improved cultivars, we have explored two diverse recombinant inbred line (RIL) populations to identify the quantitative trait loci (QTLs) associated with SPC. The two RIL populations, MP 1918 × P0540-91 (PR-30) and Ballet × Cameor (PR-31), were derived from crosses between moderate SPC × high SPC accessions. A total of 166 and 159 RILs of PR-30 and PR-31, respectively, were genotyped using an Axiom® 90K SNP array and 13.2K SNP arrays, respectively. The RILs were phenotyped in replicated trials in two and three locations of Saskatchewan, Canada in 2020 and 2021, respectively, for agronomic assessment and SPC. Using composite interval mapping, we identified three QTLs associated with SPC in PR-30 and five QTLs in PR-31, with the LOD value ranging from 3.0 to 11.0. A majority of these QTLs were unique to these populations compared to the previously known QTLs for SPC. The QTL overlapped with the earlier reported SPC associated QTL PC-QTL-3. Three QTLs, , , and with LOD scores of 7.2, 7.9, and 11.3, and which explained 14.5%, 11.6%, and 11.3% of the phenotypic variance, respectively, can be used for marker-assisted breeding to increase SPC in peas. Eight QTLs associated with the grain yield were identified with LOD scores ranging from 3.1 to 8.2. Two sets of QTLs, and , and and , shared the QTL/peak regions. Each set of QTLs contributed to either SPC or grain yield depending on which parent the QTL region is derived from, thus confirming that breeding for SPC should take into consideration the effects on grain yield.
由于消费者对植物性蛋白质的需求以及蛋白质分离行业的需求，提高豌豆（L.）种子蛋白质浓度（SPC）已成为一项重要的育种目标。为了支持通过标记辅助选择（MAS）来加速改良品种的育种进程，我们研究了两个不同的重组自交系（RIL）群体，以确定与SPC相关的数量性状位点（QTL）。这两个RIL群体，MP 1918×P0540 - 91（PR - 30）和Ballet×Cameor（PR - 31），源自中等SPC×高SPC材料之间的杂交。PR - 30和PR - 31分别有166个和159个RIL，分别使用Axiom® 90K SNP芯片和13.2K SNP芯片进行基因分型。这些RIL分别于2020年和2021年在加拿大萨斯喀彻温省的两个和三个地点进行重复试验，以进行农艺评估和SPC测定。使用复合区间作图法，我们在PR - 30中鉴定出3个与SPC相关的QTL，在PR - 31中鉴定出5个QTL，LOD值范围为3.0至11.0。与之前已知的SPC相关QTL相比，这些QTL中的大多数在这些群体中是独特的。该QTL与先前报道的与SPC相关的QTL PC - QTL - 3重叠。三个QTL， 、 和 ，LOD分数分别为7.2、7.9和11.3，分别解释了14.5%、11.6%和11.3%的表型变异，可用于标记辅助育种以提高豌豆的SPC。鉴定出8个与籽粒产量相关的QTL，LOD分数范围为3.1至8.2。两组QTL， 和 ，以及 和 ，共享QTL/峰值区域。每组QTL对SPC或籽粒产量的贡献取决于QTL区域源自哪个亲本，从而证实了SPC育种应考虑对籽粒产量的影响。
