Li Yuan, Wang Nan, Guo Jianwen, Zhou Xianjun, Bai Xueyi, Azeem Muhammad, Zhu Liyun, Chen Lin, Chu Moli, Wang Hui, Cheng Wei
College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Metabolic Diseases, Anhui Normal University, Wuhu 241000, China.
Biology (Basel). 2024 Mar 14;13(3):186. doi: 10.3390/biology13030186.
Phytophthora blight of pepper is a notorious disease caused by the oomycete pathogen , which poses a great threat to global pepper production. MicroRNA (miRNA) is a class of non-coding small RNAs that regulate gene expressions by altering the translation efficiency or stability of targeted mRNAs, which play important roles in the regulation of a plant's response to pathogens. Herein, time-series mRNA-seq libraries and small RNA-seq libraries were constructed using pepper roots from the resistant line CM334 and the susceptible line EC01 inoculated with at 0, 6, 24, and 48 h post-inoculation, respectively. For mRNA-seq analysis, a total of 2159 and 2971 differentially expressed genes (DEGs) were identified in CM334 and EC01, respectively. For miRNA-seq analysis, 491 pepper miRNAs were identified, including 330 known miRNAs and 161 novel miRNAs. Among them, 69 and 88 differentially expressed miRNAs (DEMs) were identified in CM334 and EC01, respectively. Examination of DEMs and their targets revealed 22 regulatory networks, predominantly featuring up-regulated miRNAs corresponding to down-regulated target genes. Notably, these DEM-DEG regulatory networks exhibited significant overlap between CM334 and EC01, suggesting that they might contribute to pepper's basal defense against . Furthermore, five selected DEMs (miR166, miR1171, miR395, miR530 and miRN2) and their target genes underwent qRT-PCR validation, confirming a consistent negative correlation in the expression patterns of miRNAs and their targets. This comprehensive analysis provides novel insights into the regulatory networks of miRNAs and their targets, offering valuable contributions to our understanding of pepper's defense mechanisms against .
辣椒疫霉病是由卵菌病原体引起的一种臭名昭著的病害，对全球辣椒生产构成巨大威胁。微小RNA（miRNA）是一类非编码小RNA，通过改变靶标mRNA的翻译效率或稳定性来调控基因表达，在植物对病原体反应的调控中发挥重要作用。在此，分别使用接种后0、6、24和48小时的抗性品系CM334和感病品系EC01的辣椒根构建了时间序列mRNA测序文库和小RNA测序文库。对于mRNA测序分析，在CM334和EC01中分别鉴定出2159个和2971个差异表达基因（DEG）。对于miRNA测序分析，鉴定出491个辣椒miRNA，包括330个已知miRNA和161个新miRNA。其中，在CM334和EC01中分别鉴定出69个和88个差异表达miRNA（DEM）。对DEM及其靶标的检测揭示了22个调控网络，主要特征是上调的miRNA对应下调的靶基因。值得注意的是，这些DEM-DEG调控网络在CM334和EC01之间表现出显著重叠，表明它们可能有助于辣椒对[病原体名称未给出]的基础防御。此外，对5个选定的DEM（miR166、miR1171、miR395、miR530和miRN2）及其靶基因进行了qRT-PCR验证，证实了miRNA与其靶标在表达模式上存在一致的负相关。这项综合分析为miRNA及其靶标的调控网络提供了新见解，为我们理解辣椒对[病原体名称未给出]的防御机制提供了有价值的贡献。
