Guan Yuanyuan, Wei Zhiyuan, Song Puwen, Zhou Luyi, Hu Haiyan, Hu Ping, Li Chengwei
College of Life Science and Technology, Henan Engineering Research Center of Crop Genome Editing, Henan Institute of Science and Technology, Xinxiang 453000, China.
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Phytopathology. 2023 Mar;113(3):497-507. doi: 10.1094/PHYTO-04-22-0117-R. Epub 2023 Mar 24.
Late blight and powdery mildew are two widespread tomato diseases caused by and , respectively, which reduce the quantity and quality of tomato. MicroRNAs (miRNAs) play critical roles in tomato resistance to various pathogens. Investigating the function of miRNAs is of great significance in controlling tomato diseases. To identify potential miRNAs involved in the interaction of tomato with or , we analyzed the expression profiles of small RNAs in tomato leaves infected with these two pathogens using RNA-seq technology. A total of 330 and 288 miRNAs exhibited differences in expression levels after exposure to and , respectively. One hundred and forty-six commonly differentially expressed (DE) miRNAs responsive to and infestation were detected, including 10 commonly known conserved DE miRNAs and 136 novel miRNAs. Among these known DE miRNAs, sly-miR397 was strongly downregulated in response to or infection. Silencing of sly-miR397 resulted in enhanced tolerance to the pathogens, whereas overexpression of sly-miR397 showed increased susceptibility. Furthermore, changes in sly-miR397 expression could also affect expression levels of pathogenesis-related genes and reactive oxygen species-scavenging genes, leading to altered necrotic cells and HO levels. In addition, the number of lateral branches significantly changed in transgenic plants. Taken together, our results provide potential miRNA resources for further research of miRNA-disease associations and indicates that sly-miR397 acts as a negative regulator of disease resistance and influences lateral branch development in tomato.
晚疫病和白粉病是分别由[病原体名称1]和[病原体名称2]引起的两种广泛传播的番茄病害，它们会降低番茄的产量和品质。微小RNA（miRNA）在番茄对各种病原体的抗性中起关键作用。研究miRNA的功能对于控制番茄病害具有重要意义。为了鉴定参与番茄与[病原体名称1]或[病原体名称2]相互作用的潜在miRNA，我们使用RNA测序技术分析了感染这两种病原体的番茄叶片中小RNA的表达谱。分别有330个和288个miRNA在暴露于[病原体名称1]和[病原体名称2]后表达水平出现差异。检测到146个对[病原体名称1]和[病原体名称2]侵染共同差异表达（DE）的miRNA，包括10个常见的已知保守DE miRNA和136个新的miRNA。在这些已知的DE miRNA中，sly-miR397在响应[病原体名称1]或[病原体名称2]感染时强烈下调。沉默sly-miR397导致对病原体的耐受性增强，而sly-miR397的过表达则显示易感性增加。此外，sly-miR397表达的变化也会影响病程相关基因和活性氧清除基因的表达水平，导致坏死细胞和HO水平改变。此外，转基因植物中的侧枝数量显著变化。综上所述，我们的结果为进一步研究miRNA与疾病的关联提供了潜在的miRNA资源，并表明sly-miR397作为抗病性的负调节因子，影响番茄侧枝发育。
