Cerqueira Fátima, Medeiros Rui, Lopes Inês, Campos Carla, Ferraz Maria Pia, Silva Fernando, Alves Luís G, Pinto Eugénia
FP-I3ID, FP-BHS, GIT-LoSa, University Fernando Pessoa, Praça 9 de Abril, 349, 4249-004 Porto, Portugal.
Faculty of Health Sciences, University Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal.
Antibiotics (Basel). 2024 Feb 28;13(3):222. doi: 10.3390/antibiotics13030222.
The importance of fungal infections, particularly those caused by yeasts, is increasing among the medical community. and are amongst the high-priority fungal species identified by the World Health Organization (WHO) and are considered in the critical group, while is included in the medium-priority group. The cyclam salt H[H(PhCH)Cyclam]Cl proved to be active against the growth of these three yeasts, and the aim of this work was to verify its interference with their virulence mechanisms, whether shared or unique. H[H(PhCH)Cyclam]Cl significantly inhibited biofilm production and catalase activity, being able to interfere with dimorphic transition and melanin production. At the minimal inhibitory concentration (MIC) values, H[H(PhCH)Cyclam]Cl had no antioxidant effect, as determined by the DPPH method. When using the RAW264.7 macrophage cell line, H[H(PhCH)Cyclam]Cl reduced nitric oxide (NO) detection (the Griess reaction), but this effect was associated with a significant toxic effect on the cells.
真菌感染，尤其是由酵母菌引起的感染，在医学界的重要性日益增加。 和 是世界卫生组织（WHO）确定的高优先级真菌物种，属于关键类别，而 则被列入中优先级类别。环戊胺盐H[H(PhCH)Cyclam]Cl被证明对这三种酵母菌的生长具有活性，这项工作的目的是验证其对它们毒力机制的干扰，无论是共同的还是独特的。H[H(PhCH)Cyclam]Cl显著抑制生物膜形成和过氧化氢酶活性，能够干扰 的二态转变和 的黑色素生成。在最低抑菌浓度（MIC）值下，通过DPPH法测定，H[H(PhCH)Cyclam]Cl没有抗氧化作用。当使用RAW264.7巨噬细胞系时，H[H(PhCH)Cyclam]Cl降低了一氧化氮（NO）检测（格里斯反应），但这种作用与对细胞的显著毒性作用有关。
