Hefetz Idan, Bardenstein Rita, Rotem Shahar, Zaide Galia, Bilinsky Gal, Shifman Ohad, Zimhony Oren, Aloni-Grinstein Ronit
Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 7410001, Israel.
Infectious Diseases Unit, Kaplan Medical Center Faculty of Medicine, Hebrew University of Jerusalem, Rehovot 7661041, Israel.
Antibiotics (Basel). 2024 Feb 29;13(3):231. doi: 10.3390/antibiotics13030231.
Bloodstream infections (BSI) are defined by the presence of viable bacteria or fungi, accompanied by systemic signs of infection. Choosing empirical therapy based solely on patient risk factors and prior antibiotic susceptibility test (AST) may lead to either ineffective treatment or unnecessarily broad-spectrum antibiotic exposure. In general, Clinical & Laboratory Standards Institute guideline-approved ASTs have a turnaround time of 48-72 h from sample to answer, a period that may result in a critical delay in the appropriate selection of therapy. Therefore, reducing the time required for AST is highly advantageous. We have previously shown that our novel rapid AST method, MAPt (Micro-Agar-PCR-test), accurately identifies susceptibility profiles for spiked bioterrorism agents like , and directly from whole-blood and blood culture samples, even at low bacterial levels (500 CFU/mL). This study evaluated the performance of MAPt on routine bloodstream infection (BSI), focusing on and isolates from clinical cultures, including resistant strains to some of the six tested antibiotics. Notably, MAPt yielded results exceeding 95% agreement with the standard hospital method within a significantly shorter timeframe of 6 h. These findings suggest significant potential for MAPt as a rapid and reliable BSI management tool.
血流感染（BSI）定义为存在活细菌或真菌，并伴有全身感染迹象。仅根据患者风险因素和既往抗生素敏感性试验（AST）选择经验性治疗可能导致治疗无效或不必要地使用广谱抗生素。一般来说，临床和实验室标准协会指南认可的AST从样本到结果的周转时间为48 - 72小时，这段时间可能导致在适当选择治疗方法时出现严重延误。因此，减少AST所需时间非常有利。我们之前已经表明，我们新颖的快速AST方法MAPt（微琼脂PCR试验）能够直接从全血和血培养样本中准确识别诸如 、 和 等模拟生物恐怖主义病原体的药敏谱，即使在低细菌水平（500 CFU/mL）下也能做到。本研究评估了MAPt在常规血流感染（BSI）方面的性能，重点关注从临床培养物中分离出的 和 ，包括对六种测试抗生素中的一些耐药的菌株。值得注意的是，MAPt在显著更短的6小时时间内得出的结果与标准医院方法的一致性超过95%。这些发现表明MAPt作为一种快速且可靠的BSI管理工具具有巨大潜力。
