Li Qijun, Song Mengyao, Cao Ke, Zhang Qian
Laboratory Animal Center, Chongqing Medical University, Chongqing 400016, China.
Chongqing Engineering Research Center for Rodent Laboratory Animals, Chongqing 400016, China.
Curr Issues Mol Biol. 2024 Feb 27;46(3):1799-1809. doi: 10.3390/cimb46030118.
The tumor metastasis suppressor gene CD82/KAI1 has been demonstrated to impact human trophoblast invasion and migration. Communication between trophoblasts and decidual stromal cells plays a crucial role in controlling the normal invasiveness of trophoblasts. However, whether CD82/KAI1 is involved in decidualization and what role it plays remain unclear. CD82/KAI1 demonstrates specific spatiotemporal expression patterns in stromal cells undergoing decidualization during pregnancy. This is observed in both naturally pregnant females post-implantation and pseudopregnant mice undergoing induced decidualization, as detected through in situ hybridization and immunofluorescence. CD82/KAI1 expression showed a significant time-dependent increase in cultured stromal cells after 24 and 48 h of progesterone (P) and estrogen (E) treatment. This was accompanied by a notable upregulation of decidualization markers, including cyclin D3 and PR. After transducing stromal cells with the adenovirus-overexpressing CD82/KAI1 for 48 h, the expression of cyclin D3 protein increased. Meanwhile, there was an attenuated expression of CD82/KAI1 due to an adenovirus siRNA knockdown, whereas cyclin D3 and PR expressions were not affected. Our findings suggest a potential role of CD82/KAI1 in regulating the process of decidualization, providing insights into stromal cell differentiation.
肿瘤转移抑制基因CD82/KAI1已被证明会影响人滋养层细胞的侵袭和迁移。滋养层细胞与蜕膜基质细胞之间的通讯在控制滋养层细胞的正常侵袭性方面起着关键作用。然而，CD82/KAI1是否参与蜕膜化及其发挥何种作用仍不清楚。CD82/KAI1在孕期蜕膜化的基质细胞中表现出特定的时空表达模式。通过原位杂交和免疫荧光检测发现，在植入后的自然怀孕雌性小鼠以及诱导蜕膜化的假孕小鼠中均观察到这种现象。在孕酮（P）和雌激素（E）处理24小时和48小时后，培养的基质细胞中CD82/KAI1的表达呈显著的时间依赖性增加。同时，蜕膜化标志物，包括细胞周期蛋白D3和PR显著上调。用腺病毒过表达CD82/KAI1转导基质细胞48小时后，细胞周期蛋白D3蛋白的表达增加。同时，由于腺病毒siRNA敲低，CD82/KAI1的表达减弱，而细胞周期蛋白D3和PR的表达不受影响。我们的研究结果表明CD82/KAI1在调节蜕膜化过程中具有潜在作用，为基质细胞分化提供了见解。
