Lu Kuo-Yun, Cheng Li-Ching, Hung Zheng-Ci, Chen Ze-Ying, Wang Chuang-Wei, Hou Hsin-Han
Department of Nursing, Division of Basic Medical Sciences, Chang-Gung University of Science and Technology, Taoyuan 333, Taiwan.
Department of General Surgery, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, Taiwan.
Curr Issues Mol Biol. 2024 Mar 21;46(3):2701-2712. doi: 10.3390/cimb46030170.
Inflammation and collagen-degrading enzymes' overexpression promote collagen decomposition, which affects the structural integrity of the extracellular matrix. The polysaccharide and peptide extracts of the green alga () have been proven to have anti-inflammatory, wound healing, and antioxidant effects in vivo and in vitro. However, the biological properties of the non-water-soluble components of are still unknown. In the present study, we demonstrated the higher effective anti-inflammatory functions of ethyl acetate (EA) extract than water extract up to 16-30% in LPS-induced HaCaT cells, including reducing the production of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α). Furthermore, the excellent collagen homeostasis effects from were proven by suppressing the matrix metalloproteinase-1 (MMP-1) secretion, enhancing type 1 procollagen and collagen expressions dose-dependently in WS1 cells. Moreover, using UHPLC-QTOF-MS analysis, four terpenoids, siphonaxanthin, caulerpenyne, caulerpal A, and caulerpal B, were identified and may be involved in the superior collagen homeostasis and anti-inflammatory effects of the EA extract.
炎症和胶原蛋白降解酶的过度表达会促进胶原蛋白分解，这会影响细胞外基质的结构完整性。绿藻（）的多糖和肽提取物已被证明在体内和体外具有抗炎、伤口愈合和抗氧化作用。然而，绿藻非水溶性成分的生物学特性仍不清楚。在本研究中，我们证明了在脂多糖诱导的HaCaT细胞中，绿藻乙酸乙酯（EA）提取物比水提取物具有更高的有效抗炎功能，高达16%-30%，包括减少白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子-α（TNF-α）的产生。此外，通过抑制基质金属蛋白酶-1（MMP-1）分泌，在WS1细胞中剂量依赖性地增强I型前胶原和胶原蛋白表达，证明了绿藻具有出色的胶原蛋白稳态效应。此外，通过超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF-MS）分析，鉴定出四种萜类化合物，即管藻黄素、蕨藻素、蕨藻甲素和蕨藻乙素，它们可能与绿藻EA提取物卓越的胶原蛋白稳态和抗炎作用有关。
