Ren Lu, Qin Nan, Ning Junqi, Yin Hui, Lü Hong, Zhao Xiaojun
College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, China.
Key Laboratory of Sustainable Dryland Agriculture (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Taiyuan 030031, China.
J Fungi (Basel). 2024 Feb 22;10(3):169. doi: 10.3390/jof10030169.
Chilli anthracnose is a major infectious disease of the genus . Chemical control is the primary means of controlling this disease; however, the excessive use of chemical pesticides can adversely affect ecological security and human health. Here, our aim was to explore the synergistic effects of chemical and biological pesticides in the control of chilli anthracnose. The bacterial strain LY7, which is antagonistic to the anthracnose-causing fungus , inhibited the growth of by 83.52%. Through morphological and genetic analyses, this strain was identified as . Then, the compatibility of LY7 with three common chemical fungicides was determined. The in vitro protective and therapeutic efficacies of the 1 × 10 CFU/mL (colony-forming unit/mL) bacterial solution were 66.38% and 35.18%, respectively, but both were significantly lower than those of prochloraz, the most compatible fungicide. We then conducted field efficacy trials to elucidate the best combination of prochloraz and LY7; the highest control efficiency was achieved with a suspension of 1.0 × 10 CFU/mL of LY7 mixed with 0.75 g/L prochloraz (3:7 ratio). Electron microscopy revealed the inhibitory effects of LY7 and prochloraz on mycelial growth. These results suggest that an LY7-based biofungicide can partially replace prochloraz, serving as an integrated management strategy to control chilli anthracnose.
辣椒炭疽病是辣椒属的一种主要传染病。化学防治是控制这种病害的主要手段；然而，过度使用化学农药会对生态安全和人类健康产生不利影响。在此，我们的目的是探索化学农药和生物农药在防治辣椒炭疽病方面的协同作用。对引起炭疽病的真菌具有拮抗作用的细菌菌株LY7对该真菌的生长抑制率为83.52%。通过形态学和基因分析，该菌株被鉴定为[具体菌种名称未给出]。然后，测定了LY7与三种常见化学杀菌剂的相容性。1×10 CFU/mL（菌落形成单位/毫升）细菌溶液的体外保护和治疗效果分别为66.38%和35.18%，但均显著低于相容性最佳的杀菌剂咪鲜胺。然后，我们进行了田间药效试验，以阐明咪鲜胺和LY7的最佳组合；将1.0×10 CFU/mL的LY7悬浮液与0.75 g/L咪鲜胺（3:7比例）混合时，防治效果最高。电子显微镜显示了LY7和咪鲜胺对[具体菌种名称未给出]菌丝生长的抑制作用。这些结果表明，基于LY7的生物杀菌剂可以部分替代咪鲜胺，作为控制辣椒炭疽病的综合管理策略。
