Wei Yulian, Xue Jianbin, Shi Jiangtao, Li Tong, Yuan Haisheng
Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
J Fungi (Basel). 2024 Mar 4;10(3):196. doi: 10.3390/jof10030196.
In forest ecosystems, most of the soil organic matter is derived from trees, as deadwood lignocellulose and wood-decaying basidiomycetes are the most important decomposers of lignin and cellulose. is one of the most common white-rot fungi colonizing angiosperm trees and is often found in birch deadwood but seldom in pine deadwood. To reveal the mechanism through which selects angiosperms as its preferred host trees, birch and pinewood sticks were selected for culturing for two months. The weight loss, cellulose and lignin degradation rates, activities of degrading enzymes, and transcriptome analyses of two degradation models were compared and analyzed. The results showed that -degraded birchwood with higher efficiency than pinewood. A GO enrichment analysis found that more upregulated genes related to the top 30 terms showed a molecular function related to degradation, and most genes belonged to the CAZymes family in -degraded birchwood. However, pinewood degradation did not show these phenomena. A KEGG pathway analysis also indicated that, for the same pathway, more upregulated genes were involved in birchwood degradation caused by than in pinewood degradation.
在森林生态系统中，大多数土壤有机质来自树木，因为枯木木质纤维素和腐朽木材的担子菌是木质素和纤维素最重要的分解者。 是定殖在被子植物树上最常见的白腐真菌之一，经常在桦木枯木中发现，但很少在松木枯木中发现。为了揭示 选择被子植物作为其首选寄主树的机制，选择桦木和松木枝条进行两个月的培养。比较并分析了两种降解模型的失重、纤维素和木质素降解率、降解酶活性以及转录组分析。结果表明， 降解桦木的效率高于松木。基因本体（GO）富集分析发现，与前30个术语相关的更多上调基因显示出与降解相关的分子功能，并且在 降解的桦木中，大多数基因属于碳水化合物活性酶（CAZymes）家族。然而，松木降解并未表现出这些现象。京都基因与基因组百科全书（KEGG）通路分析还表明，对于相同的通路， 引起的桦木降解中涉及的上调基因比松木降解中的更多。
