Moaness Mona, El-Sayed Sara A M, Beherei Hanan H, Mabrouk Mostafa
Refractories, Ceramics and Building Materials Department, Advanced Materials, Technology and Mineral Resources Research Institute, National Research Centre, 33 El Bohouth St., Dokki, Cairo P.O. Box 12622, Egypt.
Academy of Scientific Research and Technology (ASRT), Cairo P.O. Box 11516, Egypt.
J Funct Biomater. 2024 Feb 20;15(3):50. doi: 10.3390/jfb15030050.
Nanoporous membranes (NPMBs) have been the focus of interest of many scientists in the last decade. However, the fouling phenomenon that takes place during the implantation period blocks pores and causes failure in the local implant. In this study, alumina NPMBs were developed using electrochemical anodization through two steps. Furthermore, graphene oxide (GO), free and impregnated with ZIF-8 MOF, was synthesized and loaded in a mixture of PVDF/PVP polymer matrix at different ratios, and was applied to the produced NPMBs using spin-coater. The NPMBs were characterized before and after coating by SEM/EDX, TEM, FTIR, XRD, contact angle and AFM. The antifouling features of the NPMBs were analyzed against two different bacterial species. The prepared alumina NPMBs demonstrated homogeneous porous structures with pore sizes ranging from 36 to 39 nm. The coated layers were proven to possess microporous coatings on the surfaces of the NPMBs. The numbers of released ions (Al and Zn) from the coated NPMBs were below the allowed limits. Bovine serum albumin (BSA) uptake in artificial cerebrospinal fluid (ACSF) was impressively reduced with the presence of coating materials. In addition, the antifouling behavior of the coated NPMBs against the selected strains of bacteria was greatly enhanced compared with the pure alumina NPMBs. Finally, NPMBs' uncoated and polymer-coated membranes were tested for their ability to deliver donepezil HCl. The results reveal the downregulation of donepezil release, especially from NPMBs coated with PVDF/PVP 0.5GO. It is advised to use the current antifouling materials and techniques to overcome the limitations of the inorganic NPMBs implants.
在过去十年中，纳米多孔膜（NPMB）一直是众多科学家关注的焦点。然而，植入期间发生的污垢现象会堵塞孔隙并导致局部植入失败。在本研究中，通过两步电化学阳极氧化法制备了氧化铝NPMB。此外，合成了游离的和负载有ZIF-8金属有机框架（MOF）的氧化石墨烯（GO），并以不同比例负载于聚偏氟乙烯（PVDF）/聚乙烯吡咯烷酮（PVP）聚合物基质混合物中，然后使用旋涂机将其应用于制备的NPMB。通过扫描电子显微镜/能谱仪（SEM/EDX）、透射电子显微镜（TEM）、傅里叶变换红外光谱仪（FTIR）、X射线衍射仪（XRD）、接触角测量仪和原子力显微镜（AFM）对涂覆前后的NPMB进行了表征。针对两种不同的细菌种类分析了NPMB的抗污性能。制备的氧化铝NPMB显示出均匀的多孔结构，孔径范围为36至39纳米。经证实，涂覆层在NPMB表面具有微孔涂层。涂覆的NPMB释放的离子（铝和锌）数量低于允许限值。在有涂层材料存在的情况下，人工脑脊液（ACSF）中牛血清白蛋白（BSA）的摄取量显著降低。此外，与纯氧化铝NPMB相比，涂覆的NPMB对所选细菌菌株的抗污行为大大增强。最后，测试了NPMB的未涂覆膜和聚合物涂覆膜递送盐酸多奈哌齐的能力。结果显示多奈哌齐释放下调，尤其是来自涂覆有PVDF/PVP 0.5GO的NPMB。建议使用当前的抗污材料和技术来克服无机NPMB植入物的局限性。
