Li Zhiyi, Ur Rehman Ihtesham, Shepherd Rebecca, Douglas Timothy E L
School of Engineering, Lancaster University, Lancaster LA1 4YW, UK.
School of Medicine and Dentistry, University of Central Lancashire, Preston PR1 2HE, UK.
J Funct Biomater. 2024 Feb 21;15(3):55. doi: 10.3390/jfb15030055.
Bone tissue engineering using osteoconductive scaffolds holds promise for regeneration, with pearl powder gaining interest for its bioactive qualities. This study used freeze drying to create chitosan (CS) scaffolds with pearl/calcium phosphate (p/CaP) powders, mimicking bone tissue structurally and compositionally. Characterization included scanning electron microscopy (SEM) and mechanical testing. X-ray diffraction (XRD) Fourier-transform infrared-photoacoustic photo-acoustic sampling (FTIR-PAS), and FTIR- attenuated total reflectance (FTIR-ATR) were used to characterize p/CaP. In vitro tests covered degradation, cell activity, and SEM analysis. The scaffolds showed notable compressive strength and modulus enhancements with increasing p/CaP content. Porosity, ranging from 60% to 90%, decreased significantly at higher pearl/CaP ratios. Optimal cell proliferation and differentiation were observed with scaffolds containing up to 30 wt.% p/CaP, with 30 wt.% pearl powder and 30 wt.% p/CaP yielding the best results. In conclusion, pearl/calcium phosphate chitosan (p/CaP_CS) composite scaffolds emerged as promising biomaterials for bone tissue engineering, combining structural mimicry and favourable biological responses.
使用具有骨传导性的支架进行骨组织工程有望实现骨再生，珍珠粉因其生物活性特性而受到关注。本研究采用冷冻干燥法制备了含有珍珠/磷酸钙（p/CaP）粉末的壳聚糖（CS）支架，在结构和组成上模拟骨组织。表征方法包括扫描电子显微镜（SEM）和力学测试。利用X射线衍射（XRD）、傅里叶变换红外光声光谱（FTIR-PAS）和傅里叶变换红外衰减全反射光谱（FTIR-ATR）对p/CaP进行表征。体外测试涵盖降解、细胞活性和SEM分析。随着p/CaP含量的增加，支架的抗压强度和模量显著提高。孔隙率在60%至90%之间，在较高的珍珠/CaP比例下显著降低。在含有高达30 wt.% p/CaP的支架中观察到最佳的细胞增殖和分化，其中30 wt.%珍珠粉和30 wt.% p/CaP产生的效果最佳。总之，珍珠/磷酸钙壳聚糖（p/CaP_CS）复合支架结合了结构模拟和良好的生物学反应，成为骨组织工程中有前景的生物材料。
