Bastarache Pierre, Timani Kenan, Ben Youssef Mariem, Omakele Enock, Vickruck Jess L, Morin Pier Jr
Department of Chemistry and Biochemistry, Universite de Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, Canada.
Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, 95 Innovation Road, Fredericton, NB E3B 4Z7, Canada.
Insects. 2024 Feb 22;15(3):147. doi: 10.3390/insects15030147.
The Colorado potato beetle ( (Say)) can cause extensive damage to agricultural crops worldwide and is a significant insect pest. This insect is notorious for its ability to evade various strategies deployed to control its spread and is known for its relative ease in developing resistance against different insecticides. Various molecular levers are leveraged by for this resistance to occur, and a complete picture of the genes involved in this process is lacking. While small non-coding RNAs, including miRNAs, are differentially expressed in insects exposed to insecticides, levels of transcript coding for proteins underlying their synthesis remain to be characterized fully. The overarching objective of this work aims to fill that gap by assessing the expression of such targets in exposed to cyantraniliprole and thiamethoxam. The expression status of , , , , , , and transcripts were quantified via qRT-PCR in adult treated with low and high doses of these compounds for different lengths of time. Variation in and expression was notably observed in exposed to cyantraniliprole, while thiamethoxam exposure was associated with the modulation of and transcript levels. The down-regulation of expression in using dsRNA, followed by cyantraniliprole treatment, was associated with a reduction in the survival of insects with reduced transcript expression. Overall, this work presents the insecticide-mediated modulation of transcripts associated with small non-coding RNA processing and showcases as a target to further investigate its relevance in cyantraniliprole response.
科罗拉多马铃薯甲虫（(Say)）会对全球农作物造成广泛损害，是一种重要的害虫。这种昆虫以其能够规避为控制其传播而采取的各种策略而臭名昭著，并且以相对容易对不同杀虫剂产生抗性而闻名。其产生这种抗性利用了各种分子机制，而参与这一过程的基因全貌尚不清楚。虽然包括微小RNA（miRNA）在内的小非编码RNA在接触杀虫剂的昆虫中差异表达，但其合成所依赖的蛋白质编码转录本水平仍有待充分表征。这项工作的总体目标是通过评估在接触氰虫酰胺和噻虫嗪的[昆虫名称未明确]中此类靶标的表达来填补这一空白。通过qRT-PCR对用低剂量和高剂量这些化合物处理不同时长的成年[昆虫名称未明确]中[具体基因名称未明确]、[具体基因名称未明确]、[具体基因名称未明确]、[具体基因名称未明确]、[具体基因名称未明确]、[具体基因名称未明确]和[具体基因名称未明确]转录本的表达状态进行了定量。在接触氰虫酰胺的[昆虫名称未明确]中显著观察到[具体基因名称未明确]和[具体基因名称未明确]表达的变化，而噻虫嗪处理与[具体基因名称未明确]和[具体基因名称未明确]转录本水平的调节有关。使用dsRNA下调[昆虫名称未明确]中[具体基因名称未明确]的表达，随后进行氰虫酰胺处理，与[具体基因名称未明确]转录本表达降低的昆虫存活率降低有关。总体而言，这项工作展示了杀虫剂介导的与小非编码RNA加工相关转录本的调节，并将[具体基因名称未明确]作为一个靶点，以进一步研究其在氰虫酰胺反应中的相关性。
