Department of Chemistry and Biochemistry, University of Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, Canada.
Atlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, Canada.
Int J Mol Sci. 2017 Dec 16;18(12):2728. doi: 10.3390/ijms18122728.
The Colorado potato beetle ( (Say)) is a significant pest of potato plants that has been controlled for more than two decades by neonicotinoid imidacloprid. can develop resistance to this agent even though the molecular mechanisms underlying this resistance are not well characterized. MicroRNAs (miRNAs) are short ribonucleic acids that have been linked to response to various insecticides in several insect models. Unfortunately, the information is lacking regarding differentially expressed miRNAs following imidacloprid treatment in . In this study, next-generation sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to identify modulated miRNAs in imidacloprid-treated versus untreated . This approach identified 33 differentially expressed miRNAs between the two experimental conditions. Of interest, miR-282 and miR-989, miRNAs previously shown to be modulated by imidacloprid in other insects, and miR-100, a miRNA associated with regulation of cytochrome P450 expression, were significantly modulated in imidacloprid-treated beetles. Overall, this work presents the first report of a miRNA signature associated with imidacloprid exposure in using a high-throughput approach. It also reveals interesting miRNA candidates that potentially underly imidacloprid response in this insect pest.
科罗拉多马铃薯甲虫（(Say)）是马铃薯植株的重要害虫，二十多年来一直被新烟碱类杀虫剂吡虫啉所控制。尽管这种抗药性的分子机制尚未得到很好的描述，但它已经能够发展出抗药性。 microRNAs (miRNAs) 是短链核糖核酸，已与几种昆虫模型中对各种杀虫剂的反应有关。不幸的是，关于吡虫啉处理后 中差异表达的 miRNAs 的信息仍然缺乏。在这项研究中，使用下一代测序和定量实时聚合酶链反应 (qRT-PCR) 来鉴定吡虫啉处理与未处理的 之间被调节的 miRNAs。这种方法在两种实验条件之间鉴定了 33 个差异表达的 miRNAs。有趣的是，miR-282 和 miR-989 是先前在其他昆虫中被吡虫啉调节的 miRNAs，而 miR-100 是与细胞色素 P450 表达调控相关的 miRNAs，在吡虫啉处理的甲虫中显著调节。总的来说，这项工作首次报道了使用高通量方法与吡虫啉暴露相关的 miRNA 特征在 中。它还揭示了有趣的 miRNA 候选物，这些候选物可能是这种昆虫害虫对吡虫啉反应的基础。
